Detailed information of TA system TA188553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2072451..2073235
• Replicon: chromosome
• Accession: NZ_CP068823
• Organism: Escherichia coli strain RIVM_C029494

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JNN66_RS09680
• Protein ID: WP_000613626.1
• Coordinates: 2072451..2072945 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: JNN66_RS09685
• Protein ID: WP_000718446.1
• Coordinates: 2072942..2073235 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN66_RS09660	2067644..2068741	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
JNN66_RS09665	2068741..2069682	+	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JNN66_RS09670	2069748..2071391	+	1644	WP_000096449.1	flagellar hook-associated protein FlgK	-
JNN66_RS09675	2071403..2072356	+	954	WP_001212748.1	flagellar hook-associated protein FlgL	-
JNN66_RS09680	2072451..2072945	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JNN66_RS09685	2072942..2073235	-	294	WP_000718446.1	DUF1778 domain-containing protein	Antitoxin
JNN66_RS09690	2073369..2076542	-	3174	WP_000827394.1	ribonuclease E	-
JNN66_RS09695	2077115..2078074	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T188553 WP_000613626.1 NZ_CP068823:c2072945-2072451 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T188553 NZ_CP090506:c533827-533534 [Xylella fastidiosa subsp. morus]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACAACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11053.63 Da
• Isoelectric Point: 10.5423

>AT188553 WP_000718446.1 NZ_CP068823:c2073235-2072942 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT188553 NZ_CP090506:c534126-533830 [Xylella fastidiosa subsp. morus]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCAGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9