Detailed information of TA system TA188550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 350712..351343
• Replicon: chromosome
• Accession: NZ_CP068823
• Organism: Escherichia coli strain RIVM_C029494

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JNN66_RS01580
• Protein ID: WP_075861597.1
• Coordinates: 350712..350987 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: JNN66_RS01585
• Protein ID: WP_000593555.1
• Coordinates: 350984..351343 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN66_RS01565	345716..346783	+	1068	WP_000361481.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JNN66_RS01570	346780..349515	+	2736	WP_000149118.1	ribosome-associated ATPase/putative transporter RbbA	-
JNN66_RS01575	349515..350639	+	1125	WP_001314210.1	ABC transporter permease	-
JNN66_RS01580	350712..350987	+	276	WP_075861597.1	type II toxin-antitoxin system HicA family toxin	Toxin
JNN66_RS01585	350984..351343	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JNN66_RS01590	351393..352253	-	861	WP_001131792.1	class II aldolase	-
JNN66_RS01595	352285..352554	-	270	WP_000949581.1	HPr family phosphocarrier protein	-
JNN66_RS01600	352544..354052	-	1509	WP_012311797.1	carbohydrate kinase	-
JNN66_RS01605	354045..355403	-	1359	WP_001302220.1	PTS galactitol transporter subunit IIC	-
JNN66_RS01610	355477..355758	-	282	WP_000084021.1	PTS sugar transporter subunit IIB	-
JNN66_RS01615	355755..356228	-	474	WP_012311717.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10137.82 Da
• Isoelectric Point: 10.6128

>T188550 WP_075861597.1 NZ_CP068823:350712-350987 [Escherichia coli]
MRTQVQALRKKQKNTLDQIVKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T188550 NZ_CP090506:503141-503644 [Xylella fastidiosa subsp. morus]
ATGAAATACGCAGGGGATCGTGGTGATCCTTACCTGGACAGCGAAACAGGTGTTCTTCGTAATCTCCTTGGAATCAGGGA
TCAGGGGTGGCTCGATAAAATAGAGTCCACCCTTTCTTTTTTGCGAACCAGCGAATTGCGTGAGCGACCCGTAAAAGGCA
AATTTGATCTAGCGCATTTACAGGAAATCCATAATCGTCTTTTCCAGGATGTGTACGACTGGGCGGGTCAAATCCGTCAA
GTTGAAATCTCGAAAGGCAACACGATGTTTGCCCAGCAGATTGCGATTCAGAGTGCGGCGCAGCAGATCTTTGGGCAGCT
TGCTAAAGAGCGATTTTTGTGTGGCCTTGATGCTGAAGAATTCAGCAAACGGGCAGGTGATTATCTTGGTGAAATTAATG
TACTTCACCCATTCCGCGAGGGAAATGGCAGGACGCAGCGGGAATTCATCGCGCAATTGGCTCAGCGTGCAGGTTACCGG
ATCGACTGGGGCGTGGTCAGCTAG

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT188550 WP_000593555.1 NZ_CP068823:350984-351343 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT188550 NZ_CP090506:502956-503144 [Xylella fastidiosa subsp. morus]
ATGCTGACAGATCATGAAAAAGCTGAACGCCGTGCTGTGGTGCAAAGCGCACTCGCAAGCCAACGCATTGAGGGGTTAGA
GCCGGATGCGCAAGCCGTTGTTGATGCTGAATGCTGGGCGCGTGGTGAAATGACTATCGCTGCCGCCGTGGACCAGTACA
AAGCGCGTGTTCGGCTCCAGATGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6