Detailed information of TA system TA188536

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3869928..3870763
• Replicon: chromosome
• Accession: NZ_CP068822
• Organism: Escherichia coli strain RIVM_C029324

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q83W73
• Locus tag: JNN44_RS18465
• Protein ID: WP_000854700.1
• Coordinates: 3869928..3870305 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN44_RS18470
• Protein ID: WP_001531802.1
• Coordinates: 3870395..3870763 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN44_RS18435	3865250..3865744	+	495	WP_001059463.1	MarR family transcriptional regulator	-
JNN44_RS18440	3866179..3866517	-	339	WP_000772639.1	Arm DNA-binding domain-containing protein	-
JNN44_RS18445	3866883..3868202	+	1320	WP_000144686.1	site-specific integrase	-
JNN44_RS18450	3868295..3869149	-	855	WP_024174313.1	DUF4942 domain-containing protein	-
JNN44_RS18455	3869234..3869431	-	198	WP_000839247.1	DUF957 domain-containing protein	-
JNN44_RS18460	3869443..3869931	-	489	WP_000761726.1	hypothetical protein	-
JNN44_RS18465	3869928..3870305	-	378	WP_000854700.1	TA system toxin CbtA family protein	Toxin
JNN44_RS18470	3870395..3870763	-	369	WP_001531802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN44_RS18475	3870926..3871147	-	222	WP_000692353.1	DUF987 domain-containing protein	-
JNN44_RS18480	3871216..3871692	-	477	WP_001186199.1	RadC family protein	-
JNN44_RS18485	3871708..3872193	-	486	WP_000213723.1	antirestriction protein	-
JNN44_RS18490	3872285..3873103	-	819	WP_001234709.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / vat	3821992..3898665	76673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.28 Da
• Isoelectric Point: 7.8046

>T188536 WP_000854700.1 NZ_CP068822:c3870305-3869928 [Escherichia coli]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T188536 NZ_CP090504:c1155013-1154609 [Xylella fastidiosa subsp. morus]
GTGAGCGGCTATATGCTCGACACGAATATCATCAGCGATATCATTCGTAACCCATTCGGGGCGGTTGCTTGCCGTATTGA
ACACGTTGGGTATCCGAACATTTGCACGAGCGTTATCGTGGCGGCTGAACTTCGCTATGGATGCACGAAAAATGGCTCGG
TAAAGCTGCTGAGCAGGGTACAGGACATTCTTAAAACGCTGCCGCTCCTGCCTTTGGAAATACCTGTTGACACAACATAT
GGCAGTATCCGTGCTGAGCTGGAGGCTGCGGGTCAGCTGATCGGCGCTAATGATCTATTGATTGCTGCGCATGCTTACGT
ACTCGGGCTGACTTTGGTCACGGACAACACCCGCGAGTTCAGCCGAATTCGCGGCTTGGATGTGCAGAACTGGCTAGAGA
GGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13422.27 Da
• Isoelectric Point: 6.3161

>AT188536 WP_001531802.1 NZ_CP068822:c3870763-3870395 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNSLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188536 NZ_CP090504:c1155282-1155010 [Xylella fastidiosa subsp. morus]
ATGCCAATTTCATCTCCGTTATCACGTACCAAGGAAGCGAAGCTATTCCGAAATAATCGTAGCCAGGCGGTACGAATTCC
CGTTGAATTCGAGCTGCCGGGCGATCGTGTTCTGATCCACCGTGAGGGGAGCAAGCTCATTATTGAGCCGATCACCAGGC
CAACCAATATTGTTGAATTACTGCACGAGTGGCGAAGAGAAACACCACTCGACCCAGAAGATCAATTCCCTGATATTGAT
GACATGCCCGTTCAGCCAGAGGATATTCTGTGA

Structures

Toxin

• AlphaFold DB: A0A454A1I2