Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2530966..2532554 Replicon chromosome
Accession NZ_CP068822
Organism Escherichia coli strain RIVM_C029324

Toxin (Protein)


Gene name hipA Uniprot ID A0A192ENQ5
Locus tag JNN44_RS11895 Protein ID WP_001125432.1
Coordinates 2531232..2532554 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag JNN44_RS11890 Protein ID WP_001304368.1
Coordinates 2530966..2531232 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN44_RS11875 2527334..2528785 + 1452 WP_000854642.1 tagaturonate reductase -
JNN44_RS11880 2528982..2529896 + 915 WP_001298637.1 bestrophin family protein -
JNN44_RS11885 2529900..2530658 - 759 WP_001286538.1 trans-aconitate 2-methyltransferase -
JNN44_RS11890 2530966..2531232 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
JNN44_RS11895 2531232..2532554 + 1323 WP_001125432.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
JNN44_RS11900 2532730..2533422 - 693 WP_000382466.1 hypothetical protein -
JNN44_RS11905 2533434..2533709 - 276 WP_000948310.1 hypothetical protein -
JNN44_RS11910 2533711..2533851 - 141 WP_001335097.1 hypothetical protein -
JNN44_RS11915 2533967..2534149 - 183 WP_001335098.1 hypothetical protein -
JNN44_RS11920 2534319..2535371 + 1053 WP_000568119.1 hypothetical protein -
JNN44_RS11925 2535383..2535700 + 318 WP_000073834.1 hypothetical protein -
JNN44_RS11930 2535904..2536146 + 243 WP_000699011.1 phage capsid protein -
JNN44_RS11935 2536193..2537473 + 1281 WP_000823306.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49297.02 Da        Isoelectric Point: 8.6972

>T188530 WP_001125432.1 NZ_CP068822:2531232-2532554 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

>T188530 NZ_CP090504:503171-503674 [Xylella fastidiosa subsp. morus]
ATGAAATACGCAGGGGATCGTGGTGATCCTTACCTGGACAGCGAAACAGGTGTTCTTCGTAATCTCCTTGGAATCAGGGA
TCAGGGGTGGCTCGATAAAATAGAGTCCACCCTTTCTTTTTTGCGAACCAGCGAATTGCGTGAGCGACCCGTAAAAGGCA
AATTTGATCTAGCGCATTTACAGGAAATCCATAATCGTCTTTTCCAGGATGTGTACGACTGGGCGGGTCAAATCCGTCAA
GTTGAAATCTCGAAAGGCAACACGATGTTTGCCCAGCAGATTGCGATTCAGAGTGCGGCGCAGCAGATCTTTGGGCAGCT
TGCTAAAGAGCGATTTTTGTGTGGCCTTGATGCTGAAGAATTCAGCAAACGGGCAGGTGATTATCTTGGTGAAATTAATG
TACTTCACCCATTCCGCGAGGGAAATGGCAGGACGCAGCGGGAATTCATCGCGCAATTGGCTCAGCGTGCAGGTTACCGG
ATCGACTGGGGCGTGGTCAGCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT188530 WP_001304368.1 NZ_CP068822:2530966-2531232 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT188530 NZ_CP090504:502986-503174 [Xylella fastidiosa subsp. morus]
ATGCTGACAGATCATGAAAAAGCTGAACGCCGTGCTGTGGTGCAAAGCGCACTCGCAAGCCAACGCATTGAGGGGTTAGA
GCCGGATGCGCAAGCCGTTGTTGATGCTGAATGCTGGGCGCGTGGTGAAATGACTATCGCTGCCGCCGTGGACCAGTACA
AAGCGCGTGTTCGGCTCCAGATGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192ENQ5

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References