Detailed information of TA system TA188521

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1959601..1960432
• Replicon: chromosome
• Accession: NZ_CP068822
• Organism: Escherichia coli strain RIVM_C029324

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JNN44_RS09185
• Protein ID: WP_000854814.1
• Coordinates: 1959601..1959975 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN44_RS09190
• Protein ID: WP_024174331.1
• Coordinates: 1960064..1960432 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN44_RS09145	1954997..1956163	+	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
JNN44_RS09150	1956282..1956755	+	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
JNN44_RS09155	1956953..1958011	+	1059	WP_001200890.1	FUSC family protein	-
JNN44_RS09160	1958183..1958512	+	330	WP_000450409.1	DUF496 family protein	-
JNN44_RS09165	1958613..1958795	-	183	WP_001016348.1	hypothetical protein	-
JNN44_RS09170	1958849..1958995	+	147	Protein_1795	transposase domain-containing protein	-
JNN44_RS09175	1959284..1959397	-	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN44_RS09180	1959410..1959604	-	195	WP_000988601.1	hypothetical protein	-
JNN44_RS09185	1959601..1959975	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN44_RS09190	1960064..1960432	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN44_RS09195	1960506..1960727	-	222	WP_000692346.1	DUF987 domain-containing protein	-
JNN44_RS09200	1960796..1961272	-	477	WP_001541535.1	RadC family protein	-
JNN44_RS09205	1961288..1961761	-	474	WP_000855074.1	antirestriction protein	-
JNN44_RS09210	1962024..1962845	-	822	WP_001234571.1	DUF945 domain-containing protein	-
JNN44_RS09215	1963066..1963476	-	411	WP_000846704.1	hypothetical protein	-
JNN44_RS09220	1963492..1964169	-	678	WP_001362823.1	hypothetical protein	-
JNN44_RS09225	1964305..1965375	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T188521 WP_000854814.1 NZ_CP068822:c1959975-1959601 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T188521 NZ_CP090489:c683213-682893 [Photobacterium damselae subsp. damselae]
ATGGCTTGGAAAGTGGATTTTTACTCTGGCGTTGAAGATGAAATATTAGCAATGCCACCAAAAATTCAGGCTCGTATGAT
TAAGTTACTTGAATTAATTGAATTACATGGAGCAAATTTAGGCCCACCACATACAGAATCCATGGGTGATGGTCTTTTTG
AGATAAGAGCTAAAGCTCAAGAAGGCATAGGTAGAGGTTTGTTTTGCTATCTAAAAGGACAACACGTTTACGTATTACGA
GCTTTTGTAAAAAAGACAAATAAAACGCCCAAAAAAGAAATAGAGATCGCTAAAAAACGTATGAAAGAGGTGACGTTATG
A

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT188521 WP_024174331.1 NZ_CP068822:c1960432-1960064 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT188521 NZ_CP090489:c682896-682621 [Photobacterium damselae subsp. damselae]
ATGAGTTTATCTGAGCTAAAAAGAAAAGCACTACAAAACCCTGAGGTAAAGGAAGCTTATGACGGTCTAAGTGCTGAGTT
TGAATTGATCAGTTCTTTGATTTCAATGAGAGAACAATCAGGTTTAACTCAAGATGAAGTTGCAGCAAAAATGGGAACAA
AAGCCCCAAACATTTCACGCTTAGAATCTGGACGCTCAAACCCTAGTCTAAAAACATTAATCAGTTATGCTCAAGCATGT
GGGTTTAAACTCAATCTTGGTTTTAAACATATCTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50