Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4782146..4782643 Replicon chromosome
Accession NZ_CP068821
Organism Escherichia coli strain RIVM_C029952

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN43_RS23305 Protein ID WP_001173343.1
Coordinates 4782146..4782319 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN43_RS23310 Protein ID WP_000398619.1
Coordinates 4782347..4782643 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN43_RS23295 4778816..4780273 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
JNN43_RS23300 4780510..4782027 + 1518 WP_001550472.1 lysine--tRNA ligase -
JNN43_RS23305 4782146..4782319 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN43_RS23310 4782347..4782643 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN43_RS23315 4782871..4783143 - 273 WP_000405647.1 N-acetyltransferase -
JNN43_RS23320 4783155..4783385 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
JNN43_RS23325 4783589..4785196 + 1608 WP_000944875.1 sensor histidine kinase -
JNN43_RS23330 4785193..4785912 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4737268..4782643 45375


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T188512 WP_001173343.1 NZ_CP068821:c4782319-4782146 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T188512 NZ_CP090484:c1589775-1589494 [Burkholderia sp. S-53]
ATGACCTTTGAGCTCGCCTTTCTCGAAGCTGCGTTGAAGGAGTGGAAGAAGCTCGATCGCACCGTTCGTGATCAGTTCAA
GGCCAGGCTCGCCGAACGGCTCGAGAATCCTCGCATTCCCTCGGCGAAGTTGCACGGTCATCCGGACCGCTACAAGATCA
AGCTGTGCAGTGTCGGCTATCGCCTCGTGTACGAAGTGCGCGATGCAGAGGTCGTCGTACTGGTGGTCGAGGTAGGGCGT
CGCGAGCGCGATGCGGTCTATCTCGCCGCAATGAAACGCTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT188512 WP_000398619.1 NZ_CP068821:c4782643-4782347 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT188512 NZ_CP090484:c1590013-1589765 [Burkholderia sp. S-53]
ATGCCCCACACCATCCTGGCCAGCGTGACGGCGAGCGTCTCGGAGTTGAAGCGGAACCCGATGGGAACCGTGGCGGCGGG
AGAGGGCTTCCCGGTCGCGATCCTGAATCACAACGAACCCGCGTTCTATTGCATCCCGGCGAAGACATGGGAGGCGATGG
TCGAACGTCTTGAAGATATCGAAGACAACGCGCTGGCCGACTCGCGCGCCAAGGAGAAAGCCGTCAAGGTCAAGCTGAAT
GACCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References