Detailed information of TA system TA188507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4439247..4439755
• Replicon: chromosome
• Accession: NZ_CP068821
• Organism: Escherichia coli strain RIVM_C029952

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: JNN43_RS21455
• Protein ID: WP_001534843.1
• Coordinates: 4439546..4439755 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: JNN43_RS21450
• Protein ID: WP_000063161.1
• Coordinates: 4439247..4439549 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN43_RS21435	4435775..4437925	+	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-
JNN43_RS21440	4438043..4438246	+	204	WP_001534846.1	YbdD/YjiX family protein	-
JNN43_RS21445	4438257..4439213	+	957	WP_001534845.1	GTPase	-
JNN43_RS21450	4439247..4439549	-	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
JNN43_RS21455	4439546..4439755	-	210	WP_001534843.1	BrnT family toxin	Toxin
JNN43_RS21460	4439851..4441017	+	1167	WP_001534841.1	restriction endonuclease	-
JNN43_RS21465	4441081..4442700	+	1620	WP_001550561.1	type I restriction-modification system subunit M	-
JNN43_RS21470	4442690..4444000	+	1311	WP_001534837.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T188507 WP_001534843.1 NZ_CP068821:c4439755-4439546 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T188507 NZ_CP090484:c614584-613688 [Burkholderia sp. S-53]
ATGAGTGTCATCGACAAACGTGAACGCGACCGCACCGCGGCATTCGCCGCGCTGCTCGGGCAGTTTCCCGAGCAGCGTTG
CGCGGCCGGCGCGGCGGGCACGATCGGATATCGCGAGGCCGGCGCGCAGCATGCGGGCCGCGCGCTGCCTGTCGTGCTGC
TGCACGGGATCGGGTCGGGCGCCGCATCGTGGGTGCGCCAGCTCGACGCGCTCGGCGCATCGCGGCGCGTGCTCGCGTGG
GATGCGCCCGGTTATGGCGCGTCGACGCCCGTGCACGGCGCGTCGCCGGCCGCCGCCGATTACGCGGCGTCGCTGAACGC
GTGGCTCGAGGCACTCGGCATCGAACGCTGTGTGCTGGTCGGGCATTCGCTCGGCGCGATCGTCGCGGGCGGTCTCGCCC
GCGTGATGCCCGGGCGGATCGCCGGCCTGCTGCTGGTGTCGCCCGCGGGCGGCTACGGCAGCGCGCCCGCCGAAACGCGC
GAATCGCGGCGCGACGCGCGGCTCGCGATGCTCGCGGAACTCGGCCCGGCCGGGCTCGCCGAGCAGCGCAGCGGCAACAT
GCTGTCCGGTTTCGCGAGCGAGGATGCGCGTGCGTGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCGC
AGGCCACGCACCTGCTCGCGAACGCCGATCTCGCAACCGATCTCGCCGGTTTCCGCGGCCGCACGGCCGTGGCCGTCGGC
GCGAACGACGCAATTACGCCGCCGGCCGCCTGCGAGCGAATCGCCGCGGCCGCGCACGTCGGGCTGCAGGTGATTCCGCA
AGCCGGTCATGCCGGGTACGTGGAAGCGCCGGCCGTTTATTCCGCACTGATCGACACGTTCTGCCGTCAGTGCGATACGC
AGCGAGGGCTGGTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT188507 WP_000063161.1 NZ_CP068821:c4439549-4439247 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT188507 NZ_CP090484:c613691-612834 [Burkholderia sp. S-53]
ATGAGCGAACCGCTGCAACAACAGGAATCCGAGAACGCGAAGGCGGAAGCCAGCTACGTGGTGCCGGGCCTCGAACGCGG
GCTGCGGATTCTCGCCGAATTCTCGCCGCGCGAGCCGGTGCTCGGCGCACCCGAGTTGTCGAAGCGGCTCGGCATTCCGC
GCACGACGGTGTTCCGTCTGCTGCAGACGCTCGAATCGCTCGGCTTCCTCGAGCGCGCGGACAAGGACCGCAACTACAAG
CTCGGCATCGCCGTGCTGCGGCTCGGCTTCGAATACCTGAGCTCGCTGGAGCTGACCGATCTCGGCCTGCCGGTGATCGA
GAGCCTGCGCGACGCGACCGGCTTCACGACGCACATCGTGATCCGCGACGGCCGTGACGTCGTGTTCGTCGCGAAAGCGC
AGAGCCAGGCGCCGGTGTTCAGCTCGATCCGCGTGAACGTCGGCACCCGCTTGCCCGCGCATGCGACGACGCATGGCCAT
GTGCTGATGGGCGACCTGTCGCTGAAGGAGCTGCGCGCGCTGTATCCGCAAGGCATGCTGAACCGGATGACGAACGCGAC
GCCGGAGACGGTCGATGCGCTGTACGAAGTGATCCGCGAGGATGCGCTGCGCGGCTATGGCGTCAGCAACTCCTCGTTCG
AGCGCGGCATCTCGGTGGTGACGGCGCCCGTGCGCAACGAGACGCAGAAGATCGTCGCGTGCATCACGGTGACGGTGCCG
CGGCCGGAGATCGACGCGGCGCTGATCGCGGACGGGTTGATCGACAAGGTGCAGCGCGCGGCGGCGGAACTGTCGCGGCG
GCTCAATTACCGCTCGGATGACGAGCACACGTTTCTCAAGGCGTTAGGACTCCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3