Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3832294..3832912 Replicon chromosome
Accession NZ_CP068821
Organism Escherichia coli strain RIVM_C029952

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JNN43_RS18545 Protein ID WP_001291435.1
Coordinates 3832694..3832912 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JNN43_RS18540 Protein ID WP_000344800.1
Coordinates 3832294..3832668 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN43_RS18530 3827383..3828576 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JNN43_RS18535 3828599..3831748 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
JNN43_RS18540 3832294..3832668 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JNN43_RS18545 3832694..3832912 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JNN43_RS18550 3833084..3833635 + 552 WP_060579167.1 maltose O-acetyltransferase -
JNN43_RS18555 3833751..3834221 + 471 WP_000136192.1 YlaC family protein -
JNN43_RS18560 3834385..3835935 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JNN43_RS18565 3835977..3836330 - 354 WP_001550741.1 DUF1428 family protein -
JNN43_RS18575 3836709..3837020 + 312 WP_000409911.1 MGMT family protein -
JNN43_RS18580 3837051..3837623 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T188504 WP_001291435.1 NZ_CP068821:3832694-3832912 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T188504 NZ_CP090484:198449-198862 [Burkholderia sp. S-53]
ATGAGCGGGATTGAGTCGGCGAAATCGTTCGTCGACAGCAATGTCGTGCTTTATCTGCTGTCCGAGGACGAAACCAAGGC
GGATCGGGCGGAGGCGCTGTTCCTTCGGCGGCCGACCATCAGTGTCCAGGTACTGAACGAAGTCGCCAGCGTGTGCAGTC
GAAAATTGCGGATGCCGTGGAGCGATGTGGGGCAGTTTCTGGAACCGATCAGGAGCTTGTGCCATGTCGTACCGGTCACG
ATCGATACGCATGACCTGGCGCGAAGGCTCGCGGAACGGCATGGCTTGTCCTTCTATGACGCGTGCATCGTCGCCGCCGC
GTCGCTCGAGGGCTGCGAGTACCTGTACACCGAGGACATGCACGACGGCCTGCGCGTGGACGGCGGTCCGGTACTGCGAA
ATCCGTTTGCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT188504 WP_000344800.1 NZ_CP068821:3832294-3832668 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT188504 NZ_CP090484:198229-198462 [Burkholderia sp. S-53]
ATGCAGGTCGCAAAGTGGGGCAATAGCCTGGCCGTCCGCCTTCCCGCCAGTGTGGTCGATGCACTGGAGCTCCGTGAAGG
GGACGATATCGAAATCGTGGTCGATAGCCCGCGCGTTTTTGCCGTCAGCCGCAAGCCGCGGGCCGATGAACTTCTGCAAC
GGTTGCGGCGTTTCAGGGGCAAGCTGCCCGCCGATTTCAAGTTCAGCCGGGACGACGCGAATGAGCGGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References