Detailed information of TA system TA188495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2973955..2975543
• Replicon: chromosome
• Accession: NZ_CP068821
• Organism: Escherichia coli strain RIVM_C029952

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: JNN43_RS14280
• Protein ID: WP_001551128.1
• Coordinates: 2973955..2975277 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JNN43_RS14285
• Protein ID: WP_001296726.1
• Coordinates: 2975277..2975543 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN43_RS14260	2969443..2969751	-	309	WP_001221046.1	hypothetical protein	-
JNN43_RS14265	2969890..2970789	+	900	WP_001551125.1	LysR family transcriptional regulator	-
JNN43_RS14270	2971156..2972188	+	1033	Protein_2792	ISNCY family transposase	-
JNN43_RS14275	2972403..2973755	+	1353	WP_001551127.1	SIR2 family protein	-
JNN43_RS14280	2973955..2975277	-	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JNN43_RS14285	2975277..2975543	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JNN43_RS14290	2975727..2977127	-	1401	WP_032146881.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2971370..2972188	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T188495 WP_001551128.1 NZ_CP068821:c2975277-2973955 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T188495 NZ_CP090475:942480-942686 [Staphylococcus haemolyticus]
ATGAACTTTAGTGAAATTAGAATTAGTGAATTAAATACTGAAGAGCAAGCTAATTCACTCCAACATAGACTTGAGAAGAT
GATAGGTGTAGATGAAGTAATTGTAGAACTAAATGACCAATTAGTTAAAATTTCTTATGAAACACCTACGAATTTAAATA
GTATTGAAAAAGAAATTTATGATGATGGTTATAAGGTATTAGGTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT188495 WP_001296726.1 NZ_CP068821:c2975543-2975277 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT188495 NZ_CP090475:942175-942468 [Staphylococcus haemolyticus]
TTGAATAATCATGAAAATGCACATCATTCCGATCAAATTAAAATGAATTTAAAATCAAGACTTAATCGTATTGAAGGGCA
AGTTAGAGCGATTAATCGCATGATTGAAGAAGATGTATACTGTGATGACGTGCTAACTCAAATTAGAGCTACAAGATCAG
CTTTAAATAGTGTGGCCACTAAGTTGCTAGATCATCATATGAAAAGCTGTATCATGGATAAAGTGAATAACGGCGCTCAA
GAAGAAGCGATGGAAGAATTGTTAGTGACTTTTCAAAAATTAATGAAAGATTAG

Structures

Toxin

• AlphaFold DB: A0A829LC11