Detailed information of TA system TA188486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2184248..2184953
• Replicon: chromosome
• Accession: NZ_CP068821
• Organism: Escherichia coli strain RIVM_C029952

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JNN43_RS10420
• Protein ID: WP_000539521.1
• Coordinates: 2184567..2184953 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JNN43_RS10415
• Protein ID: WP_001280945.1
• Coordinates: 2184248..2184577 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN43_RS10400	2179405..2180316	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
JNN43_RS10405	2180494..2182842	+	2349	WP_001550886.1	EAL domain-containing protein	-
JNN43_RS10410	2182850..2184178	+	1329	WP_001550887.1	GGDEF domain-containing protein	-
JNN43_RS10415	2184248..2184577	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JNN43_RS10420	2184567..2184953	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN43_RS10425	2185179..2186504	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JNN43_RS10430	2186717..2187100	+	384	WP_000497137.1	biofilm formation regulator BssR	-
JNN43_RS10435	2187211..2188326	+	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
JNN43_RS10440	2188323..2188949	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T188486 WP_000539521.1 NZ_CP068821:c2184953-2184567 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T188486 NZ_CP090468:4064862-4065080 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT188486 WP_001280945.1 NZ_CP068821:c2184577-2184248 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT188486 NZ_CP090468:4064461-4064835 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6