Detailed information of TA system TA188482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1117061..1117644
• Replicon: chromosome
• Accession: NZ_CP068821
• Organism: Escherichia coli strain RIVM_C029952

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: JNN43_RS05325
• Protein ID: WP_000254738.1
• Coordinates: 1117309..1117644 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: JNN43_RS05320
• Protein ID: WP_000581937.1
• Coordinates: 1117061..1117309 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN43_RS05310	1113400..1114701	+	1302	WP_001551563.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
JNN43_RS05315	1114749..1116983	+	2235	WP_001551562.1	GTP diphosphokinase	-
JNN43_RS05320	1117061..1117309	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JNN43_RS05325	1117309..1117644	+	336	WP_000254738.1	endoribonuclease MazF	Toxin
JNN43_RS05330	1117716..1118507	+	792	WP_001071643.1	nucleoside triphosphate pyrophosphohydrolase	-
JNN43_RS05335	1118735..1120372	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
JNN43_RS05340	1120460..1121758	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T188482 WP_000254738.1 NZ_CP068821:1117309-1117644 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T188482 NZ_CP090468:855500-855910 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT188482 WP_000581937.1 NZ_CP068821:1117061-1117309 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT188482 NZ_CP090468:855253-855519 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4