Detailed information of TA system TA188480

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 875986..876818
• Replicon: chromosome
• Accession: NZ_CP068821
• Organism: Escherichia coli strain RIVM_C029952

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3P5UBZ5
• Locus tag: JNN43_RS04165
• Protein ID: WP_001094454.1
• Coordinates: 875986..876360 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3G8RJV8
• Locus tag: JNN43_RS04170
• Protein ID: WP_001320394.1
• Coordinates: 876450..876818 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN43_RS04135	871099..872247	-	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-
JNN43_RS04140	872319..873302	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
JNN43_RS04145	874113..874283	-	171	Protein_813	IS110 family transposase	-
JNN43_RS04150	874626..875195	-	570	WP_001290250.1	DUF4942 domain-containing protein	-
JNN43_RS04155	875292..875489	-	198	WP_000839276.1	DUF957 domain-containing protein	-
JNN43_RS04160	875501..875989	-	489	WP_000777545.1	hypothetical protein	-
JNN43_RS04165	875986..876360	-	375	WP_001094454.1	TA system toxin CbtA family protein	Toxin
JNN43_RS04170	876450..876818	-	369	WP_001320394.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN43_RS04175	876868..877511	-	644	Protein_819	antitoxin of toxin-antitoxin stability system	-
JNN43_RS04180	877530..877751	-	222	WP_000692300.1	DUF987 domain-containing protein	-
JNN43_RS04185	877814..878290	-	477	WP_001186726.1	RadC family protein	-
JNN43_RS04190	878306..878791	-	486	WP_029701480.1	antirestriction protein	-
JNN43_RS04195	878846..879664	-	819	WP_001234642.1	DUF945 domain-containing protein	-
JNN43_RS04200	879764..879997	-	234	WP_001119719.1	DUF905 family protein	-
JNN43_RS04205	880076..880531	-	456	WP_000581493.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14059.10 Da
• Isoelectric Point: 9.2447

>T188480 WP_001094454.1 NZ_CP068821:c876360-875986 [Escherichia coli]
MNTLPDTHVRKASRCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T188480 NZ_CP068821:c876360-875986 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGAAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13623.22 Da
• Isoelectric Point: 6.4783

>AT188480 WP_001320394.1 NZ_CP068821:c876818-876450 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPSTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADANHLDQAFPLLMKQLELMFTSS
ELNPHRQNTVTLYAKGLTCHADTLGSCGYVYLAVYPTPETKQ

Nucleotide

• Length: 369 bp

>AT188480 NZ_CP068821:c876818-876450 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACTCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCAGCACCGTGAC
GCCCTGTTTCGGGGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAAGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGTTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGCTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTTGCTGTTTATCCGACGCCCGAAACGAAACAGTAA

Structures

Toxin

• AlphaFold DB: A0A3P5UBZ5

Antitoxin

• AlphaFold DB: A0A3G8RJV8