Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4197400..4198235 Replicon chromosome
Accession NZ_CP068820
Organism Escherichia coli strain RIVM_C029951

Toxin (Protein)


Gene name yeeV Uniprot ID A0A3U1IU51
Locus tag JNN41_RS20245 Protein ID WP_062878386.1
Coordinates 4197400..4197777 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3P1Z1K5
Locus tag JNN41_RS20250 Protein ID WP_021538665.1
Coordinates 4197867..4198235 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN41_RS20220 4193472..4194455 - 984 WP_024181915.1 KpsF/GutQ family sugar-phosphate isomerase -
JNN41_RS20225 4195266..4195436 - 171 Protein_3951 IS110 family transposase -
JNN41_RS20230 4195767..4196621 - 855 WP_202859627.1 DUF4942 domain-containing protein -
JNN41_RS20235 4196706..4196903 - 198 WP_000839291.1 DUF957 domain-containing protein -
JNN41_RS20240 4196915..4197403 - 489 WP_059342216.1 hypothetical protein -
JNN41_RS20245 4197400..4197777 - 378 WP_062878386.1 TA system toxin CbtA family protein Toxin
JNN41_RS20250 4197867..4198235 - 369 WP_021538665.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JNN41_RS20255 4198310..4198531 - 222 WP_001825901.1 DUF987 domain-containing protein -
JNN41_RS20260 4198594..4199070 - 477 WP_001186727.1 RadC family protein -
JNN41_RS20265 4199086..4199571 - 486 WP_021566656.1 antirestriction protein -
JNN41_RS20270 4199663..4200481 - 819 WP_001234693.1 DUF945 domain-containing protein -
JNN41_RS20275 4200582..4200815 - 234 WP_021538212.1 DUF905 family protein -
JNN41_RS20280 4200821..4201498 - 678 WP_001097364.1 hypothetical protein -
JNN41_RS20285 4201617..4202501 - 885 WP_000010387.1 50S ribosome-binding GTPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14164.14 Da        Isoelectric Point: 7.2922

>T188473 WP_062878386.1 NZ_CP068820:c4197777-4197400 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNDITLGKHPEAKQ

Download         Length: 378 bp

>T188473 NZ_CP090462:c5398226-5397843 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13465.18 Da        Isoelectric Point: 6.2015

>AT188473 WP_021538665.1 NZ_CP068820:c4198235-4197867 [Escherichia coli]
VSDTFSGTTHPDDNRGRPWWGLPCTVTPCFGARLVQEGNQLHYLADHAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGCVYLAVYPTPETKK

Download         Length: 369 bp

>AT188473 NZ_CP090462:c5398468-5398226 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3U1IU51

Antitoxin

Source ID Structure
AlphaFold DB A0A3P1Z1K5

References