Detailed information of TA system TA188471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3524149..3524761
• Replicon: chromosome
• Accession: NZ_CP068820
• Organism: Escherichia coli strain RIVM_C029951

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: JNN41_RS17000
• Protein ID: WP_000833473.1
• Coordinates: 3524149..3524334 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: JNN41_RS17005
• Protein ID: WP_000499744.1
• Coordinates: 3524351..3524761 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN41_RS16990	3520990..3522141	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
JNN41_RS16995	3522342..3523880	+	1539	WP_001551836.1	aldehyde dehydrogenase AldB	-
JNN41_RS17000	3524149..3524334	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
JNN41_RS17005	3524351..3524761	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JNN41_RS17010	3524833..3526797	-	1965	WP_001551835.1	glycoside hydrolase family 127 protein	-
JNN41_RS17015	3526808..3528208	-	1401	WP_000204811.1	MFS transporter	-
JNN41_RS17020	3528434..3529249	+	816	WP_000891823.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T188471 WP_000833473.1 NZ_CP068820:3524149-3524334 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T188471 NZ_CP090462:c4917115-4916831 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT188471 WP_000499744.1 NZ_CP068820:3524351-3524761 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT188471 NZ_CP090462:c4917347-4917105 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9