Detailed information of TA system TA188464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 2475576..2476396
• Replicon: chromosome
• Accession: NZ_CP068820
• Organism: Escherichia coli strain RIVM_C029951

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: A0A0V9MKL8
• Locus tag: JNN41_RS11975
• Protein ID: WP_001550554.1
• Coordinates: 2475576..2475833 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: JNN41_RS11980
• Protein ID: WP_001519303.1
• Coordinates: 2475845..2476396 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN41_RS11955	2470862..2471968	+	1107	WP_001519308.1	N-acetylneuraminate epimerase	-
JNN41_RS11960	2472033..2473013	+	981	WP_001519307.1	transposase	-
JNN41_RS11965	2473596..2474837	-	1242	Protein_2346	helicase YjhR	-
JNN41_RS11970	2474900..2475199	+	300	WP_001519304.1	GNAT family N-acetyltransferase	-
JNN41_RS11975	2475576..2475833	+	258	WP_001550554.1	hypothetical protein	Toxin
JNN41_RS11980	2475845..2476396	+	552	WP_001519303.1	N-acetyltransferase	Antitoxin
JNN41_RS11985	2476448..2476609	+	162	WP_001519302.1	hypothetical protein	-
JNN41_RS11990	2476646..2477194	+	549	WP_001519301.1	hypothetical protein	-
JNN41_RS11995	2477321..2477581	+	261	WP_000084876.1	hypothetical protein	-
JNN41_RS12000	2477619..2477735	+	117	Protein_2353	VOC family protein	-
JNN41_RS12005	2477980..2479101	+	1122	WP_000010833.1	M42 family metallopeptidase	-
JNN41_RS12010	2479098..2479376	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
JNN41_RS12015	2479388..2480701	+	1314	WP_001519300.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	2466986..2476609	9623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9744.33 Da
• Isoelectric Point: 11.0090

>T188464 WP_001550554.1 NZ_CP068820:2475576-2475833 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKPVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T188464 NZ_CP090462:355421-355768 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT188464 WP_001519303.1 NZ_CP068820:2475845-2476396 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT188464 NZ_CP090462:355768-356067 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0V9MKL8