Detailed information of TA system TA188463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2431913..2432421
• Replicon: chromosome
• Accession: NZ_CP068820
• Organism: Escherichia coli strain RIVM_C029951

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: JNN41_RS11770
• Protein ID: WP_001534843.1
• Coordinates: 2432212..2432421 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: JNN41_RS11765
• Protein ID: WP_000063161.1
• Coordinates: 2431913..2432215 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN41_RS11750	2428441..2430591	+	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-
JNN41_RS11755	2430709..2430912	+	204	WP_001534846.1	YbdD/YjiX family protein	-
JNN41_RS11760	2430923..2431879	+	957	WP_001534845.1	GTPase	-
JNN41_RS11765	2431913..2432215	-	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
JNN41_RS11770	2432212..2432421	-	210	WP_001534843.1	BrnT family toxin	Toxin
JNN41_RS11775	2432517..2433683	+	1167	WP_001534841.1	restriction endonuclease	-
JNN41_RS11780	2433747..2435366	+	1620	WP_001550561.1	type I restriction-modification system subunit M	-
JNN41_RS11785	2435356..2436666	+	1311	WP_001534837.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T188463 WP_001534843.1 NZ_CP068820:c2432421-2432212 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T188463 NZ_CP090462:329450-329818 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT188463 WP_000063161.1 NZ_CP068820:c2432215-2431913 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT188463 NZ_CP090462:329232-329453 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3