Detailed information of TA system TA188456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 739388..740976
• Replicon: chromosome
• Accession: NZ_CP068820
• Organism: Escherichia coli strain RIVM_C029951

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: JNN41_RS03925
• Protein ID: WP_001551128.1
• Coordinates: 739654..740976 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JNN41_RS03920
• Protein ID: WP_001296726.1
• Coordinates: 739388..739654 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN41_RS03920	739388..739654	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JNN41_RS03925	739654..740976	+	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JNN41_RS03930	741176..742528	-	1353	WP_001551127.1	SIR2 family protein	-
JNN41_RS03935	742743..743775	-	1033	Protein_775	ISNCY family transposase	-
JNN41_RS03940	744142..745041	-	900	WP_001551125.1	LysR family transcriptional regulator	-
JNN41_RS03945	745180..745488	+	309	WP_001221046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	742743..743561	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T188456 WP_001551128.1 NZ_CP068820:739654-740976 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T188456 NZ_CP090456:c4123602-4123261 [Escherichia coli]
ATGAAAACTCTACCTGCAACAATTTCGCGGGCGACGAAGCCCTGTCTGTCGCCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACATTGGATGACACGGCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCCGGGATTACCCTGGCCGATGCAGTCAACTTCTTGGTAGATAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGTTGGCAGGAGCAATCACCTTATCTTCGCGCGGTAGATATACTGCGAGCACGACGGGCAATTGGTTTATTGCGACGAAG
TATTAATGATGCTGTATTGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT188456 WP_001296726.1 NZ_CP068820:739388-739654 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT188456 NZ_CP090456:c4123940-4123623 [Escherichia coli]
ATGACTCACAACACATGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGCGGAACCGGCT
GCATTATCTGGCTGACCGGGCCAGTATTACCGGTAAATTCAGTGACGCCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGCTGAACTCAGCCCCCAACATGCCCACTGCGTCATCCTGTATCAC
AACGGTTTCACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTATGTATATATCGCAGTCTATCCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A829LC11