Detailed information of TA system TA188444

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4677857..4678691
• Replicon: chromosome
• Accession: NZ_CP068819
• Organism: Escherichia coli strain RIVM_C029042

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: JNN65_RS22680
• Protein ID: WP_000854820.1
• Coordinates: 4678314..4678691 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: JNN65_RS22675
• Protein ID: WP_001285610.1
• Coordinates: 4677857..4678225 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN65_RS22640	4673578..4674258	+	681	WP_021515201.1	WYL domain-containing protein	-
JNN65_RS22645	4674409..4675086	+	678	WP_060579144.1	hypothetical protein	-
JNN65_RS22650	4675092..4675325	+	234	WP_000883181.1	DUF905 family protein	-
JNN65_RS22655	4675415..4676233	+	819	WP_001175155.1	DUF945 domain-containing protein	-
JNN65_RS22660	4676499..4676978	+	480	WP_000706978.1	antirestriction protein	-
JNN65_RS22665	4676993..4677469	+	477	WP_001186193.1	RadC family protein	-
JNN65_RS22670	4677556..4677777	+	222	WP_000692347.1	DUF987 domain-containing protein	-
JNN65_RS22675	4677857..4678225	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN65_RS22680	4678314..4678691	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN65_RS22685	4678688..4678885	+	198	Protein_4442	hypothetical protein	-
JNN65_RS22690	4678913..4679110	+	198	WP_085949158.1	DUF957 domain-containing protein	-
JNN65_RS22695	4679195..4679755	+	561	Protein_4444	DUF4942 domain-containing protein	-
JNN65_RS22700	4680590..4682128	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4644567..4689941	45374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T188444 WP_000854820.1 NZ_CP068819:4678314-4678691 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T188444 NZ_CP090456:1050783-1051118 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT188444 WP_001285610.1 NZ_CP068819:4677857-4678225 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188444 NZ_CP090456:1050535-1050783 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6