Detailed information of TA system TA188435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3058461..3059245
• Replicon: chromosome
• Accession: NZ_CP068819
• Organism: Escherichia coli strain RIVM_C029042

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JNN65_RS14860
• Protein ID: WP_000613626.1
• Coordinates: 3058751..3059245 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: JNN65_RS14855
• Protein ID: WP_001110447.1
• Coordinates: 3058461..3058754 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN65_RS14845	3053611..3054570	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
JNN65_RS14850	3055143..3058328	+	3186	WP_001550951.1	ribonuclease E	-
JNN65_RS14855	3058461..3058754	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
JNN65_RS14860	3058751..3059245	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JNN65_RS14865	3059340..3060293	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
JNN65_RS14870	3060305..3061948	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
JNN65_RS14875	3062014..3062955	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JNN65_RS14880	3062955..3064052	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T188435 WP_000613626.1 NZ_CP068819:3058751-3059245 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T188435 NZ_CP090448:c4383281-4382970 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT188435 WP_001110447.1 NZ_CP068819:3058461-3058754 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT188435 NZ_CP090448:c4382969-4382679 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAAGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTACTG
CGAGCGATTGCCAATGATCCGCAACATGTACTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6