Detailed information of TA system TA188434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2680657..2681295
• Replicon: chromosome
• Accession: NZ_CP068819
• Organism: Escherichia coli strain RIVM_C029042

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: JNN65_RS12960
• Protein ID: WP_000813795.1
• Coordinates: 2681119..2681295 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JNN65_RS12955
• Protein ID: WP_076797675.1
• Coordinates: 2680657..2681073 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN65_RS12935	2675809..2676750	-	942	WP_001251315.1	ABC transporter permease	-
JNN65_RS12940	2676751..2677764	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
JNN65_RS12945	2677782..2678927	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
JNN65_RS12950	2679172..2680578	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
JNN65_RS12955	2680657..2681073	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JNN65_RS12960	2681119..2681295	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JNN65_RS12965	2681517..2681747	+	231	WP_023910283.1	YncJ family protein	-
JNN65_RS12970	2681839..2683800	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JNN65_RS12975	2683873..2684409	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
JNN65_RS12980	2684462..2685673	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T188434 WP_000813795.1 NZ_CP068819:c2681295-2681119 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T188434 NZ_CP090448:4018764-4019114 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCAGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT188434 WP_076797675.1 NZ_CP068819:c2681073-2680657 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT188434 NZ_CP090448:4018519-4018770 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1