Detailed information of TA system TA188424

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2031306..2032137
• Replicon: chromosome
• Accession: NZ_CP068819
• Organism: Escherichia coli strain RIVM_C029042

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JNN65_RS09800
• Protein ID: WP_000854814.1
• Coordinates: 2031306..2031680 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: JNN65_RS09805
• Protein ID: WP_001285585.1
• Coordinates: 2031769..2032137 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN65_RS09760	2026702..2027868	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
JNN65_RS09765	2027987..2028460	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
JNN65_RS09770	2028658..2029716	+	1059	WP_001200891.1	FUSC family protein	-
JNN65_RS09775	2029888..2030217	+	330	WP_000450409.1	DUF496 family protein	-
JNN65_RS09780	2030318..2030500	-	183	WP_001016348.1	hypothetical protein	-
JNN65_RS09785	2030572..2030700	+	129	Protein_1916	transposase domain-containing protein	-
JNN65_RS09790	2030989..2031102	-	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN65_RS09795	2031115..2031309	-	195	WP_000988600.1	hypothetical protein	-
JNN65_RS09800	2031306..2031680	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN65_RS09805	2031769..2032137	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
JNN65_RS09810	2032211..2032432	-	222	WP_000692323.1	DUF987 domain-containing protein	-
JNN65_RS09815	2032495..2032971	-	477	WP_039267628.1	RadC family protein	-
JNN65_RS09820	2032987..2033466	-	480	WP_000860087.1	antirestriction protein	-
JNN65_RS09825	2033548..2034366	-	819	WP_060626401.1	DUF945 domain-containing protein	-
JNN65_RS09830	2034466..2034699	-	234	WP_001119729.1	DUF905 family protein	-
JNN65_RS09835	2034774..2035229	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T188424 WP_000854814.1 NZ_CP068819:c2031680-2031306 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T188424 NZ_CP090448:c2440788-2440612 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGTGTCACCCTAGCGATGAGATTAAAGAACCATTGCGTAAAGCAATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT188424 WP_001285585.1 NZ_CP068819:c2032137-2031769 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT188424 NZ_CP090448:c2440566-2440150 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60