Detailed information of TA system TA188417

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 73358..74193
• Replicon: chromosome
• Accession: NZ_CP068819
• Organism: Escherichia coli strain RIVM_C029042

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JNN65_RS00315
• Protein ID: WP_001565141.1
• Coordinates: 73358..73735 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN65_RS00320
• Protein ID: WP_001565140.1
• Coordinates: 73825..74193 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN65_RS00285	68725..69648	-	924	WP_000535949.1	carboxylate/amino acid/amine transporter	-
JNN65_RS00290	69759..70943	-	1185	WP_001172876.1	sugar efflux transporter	-
JNN65_RS00295	71371..71553	-	183	WP_177309578.1	hypothetical protein	-
JNN65_RS00300	71737..72579	-	843	WP_001565143.1	DUF4942 domain-containing protein	-
JNN65_RS00305	72664..72861	-	198	WP_000839256.1	DUF957 domain-containing protein	-
JNN65_RS00310	72873..73361	-	489	WP_001565142.1	hypothetical protein	-
JNN65_RS00315	73358..73735	-	378	WP_001565141.1	TA system toxin CbtA family protein	Toxin
JNN65_RS00320	73825..74193	-	369	WP_001565140.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN65_RS00325	74268..74489	-	222	WP_000692303.1	DUF987 domain-containing protein	-
JNN65_RS00330	74558..75034	-	477	WP_001186724.1	RadC family protein	-
JNN65_RS00335	75050..75535	-	486	WP_001565139.1	antirestriction protein	-
JNN65_RS00340	75627..76445	-	819	WP_021536653.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14246.27 Da
• Isoelectric Point: 7.2920

>T188417 WP_001565141.1 NZ_CP068819:c73735-73358 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SICPRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T188417 NZ_CP090448:690661-691044 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13604.41 Da
• Isoelectric Point: 6.7389

>AT188417 WP_001565140.1 NZ_CP068819:c74193-73825 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFALLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188417 NZ_CP090448:690203-690571 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA