Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4763861..4764358 Replicon chromosome
Accession NZ_CP068818
Organism Escherichia coli strain RIVM_C028803

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN48_RS23400 Protein ID WP_001173343.1
Coordinates 4763861..4764034 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN48_RS23405 Protein ID WP_000398619.1
Coordinates 4764062..4764358 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN48_RS23390 4760531..4761988 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
JNN48_RS23395 4762225..4763742 + 1518 WP_001550472.1 lysine--tRNA ligase -
JNN48_RS23400 4763861..4764034 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN48_RS23405 4764062..4764358 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN48_RS23410 4764587..4764859 - 273 WP_000405647.1 N-acetyltransferase -
JNN48_RS23415 4764871..4765101 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
JNN48_RS23420 4765281..4766912 + 1632 WP_001216477.1 sensor histidine kinase -
JNN48_RS23425 4766909..4767628 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4752630..4764358 11728


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T188415 WP_001173343.1 NZ_CP068818:c4764034-4763861 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T188415 NZ_CP090447:4311965-4312387 [Streptomyces spinosirectus]
GTGAACGCCGCGCAGTACCTGATCGACACCAGCGCGCTCGCTCGTATGCTGCGCGGCGACGCCGAGCAGTACGGATGGGA
CCAGGCGGCCGCCGCCGGGCTCATCGCCACCTGCCCCATCACCGAGCTTGAGTTCTTTTGCAGTGCGCGTTCCGCCGCCG
ACCGTGCTCAAGGCATCGATGACATGCGCCTGCTCTTCGGCGGGGTGCCGGTCGACGACCGCGCCTACGACCGCGCCTGG
CGGGTCCAGGAGGTCCTCACTCAGCGCGGGCAGCACCGGAGTGCCGGAGCCGTCGATCTCGTGGTGGCTGCCACGGCCGA
GTTGCAGGGACTGACGCTGCTGCACCGTGACCGGGACTTCGAATGCATCGCCGCCGTCACCGGCCAGGCACTCCAGTGGT
ACGGCCCTGAAGCCGGCAAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT188415 WP_000398619.1 NZ_CP068818:c4764358-4764062 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT188415 NZ_CP090447:4311753-4311968 [Streptomyces spinosirectus]
ATGAGCCGAACCGTGATCGACCTCGACGACCAGCTGGTCGCTGACGTGGCCAAGGCCCTCGGTACCAGCACCAAGAAGGA
GACGGTGAACACCGCCCTGCGCGAGGTGCTGGAGAACCGGCGGCGAGCCCTGGCGCTCACCCGCCTGCGGGCGTCGGCGG
AAGAGGGTGCCTTCGACCTCGATCTCTTCGAGGACAAGGGAAACTACCGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References