Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4627433..4628028 Replicon chromosome
Accession NZ_CP068818
Organism Escherichia coli strain RIVM_C028803

Toxin (Protein)


Gene name chpB Uniprot ID A0A0V9NWK6
Locus tag JNN48_RS22635 Protein ID WP_019842229.1
Coordinates 4627433..4627783 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID L4JJX7
Locus tag JNN48_RS22640 Protein ID WP_001223213.1
Coordinates 4627777..4628028 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN48_RS22615 4622763..4623785 - 1023 WP_001550507.1 ABC transporter permease -
JNN48_RS22620 4623799..4625301 - 1503 WP_001550506.1 sugar ABC transporter ATP-binding protein -
JNN48_RS22625 4625433..4626389 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
JNN48_RS22630 4626699..4627229 + 531 WP_000055075.1 inorganic diphosphatase -
JNN48_RS22635 4627433..4627783 - 351 WP_019842229.1 endoribonuclease toxin ChpB Toxin
JNN48_RS22640 4627777..4628028 - 252 WP_001223213.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
JNN48_RS22645 4628319..4629314 - 996 WP_001372721.1 tyrosine-type recombinase/integrase -
JNN48_RS22650 4629381..4629680 - 300 WP_062897648.1 helix-turn-helix transcriptional regulator -
JNN48_RS22655 4629789..4630214 + 426 WP_062897647.1 hypothetical protein -
JNN48_RS22660 4630252..4630602 + 351 WP_062897646.1 DUF4761 family protein -
JNN48_RS22665 4630606..4630863 + 258 WP_062897645.1 hypothetical protein -
JNN48_RS22670 4630885..4631127 + 243 WP_062897644.1 DUF4754 family protein -
JNN48_RS22675 4631124..4631237 + 114 WP_062897643.1 hypothetical protein -
JNN48_RS22680 4631324..4631527 + 204 WP_000985157.1 hypothetical protein -
JNN48_RS22685 4631524..4631763 + 240 WP_158124564.1 MarR family winged helix-turn-helix transcriptional regulator -
JNN48_RS22690 4631760..4632059 + 300 WP_202847203.1 hypothetical protein -
JNN48_RS22695 4632074..4632625 + 552 WP_202847204.1 DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4627433..4663876 36443


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-115)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12483.44 Da        Isoelectric Point: 5.6097

>T188414 WP_019842229.1 NZ_CP068818:c4627783-4627433 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVWMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Download         Length: 351 bp

>T188414 NZ_CP090445:c5615233-5614355 [Comamonas testosteroni]
ATGCATGAAGATTTGCTGTCGCTGAAACTGCAGCTGCTGCAGACCGGTTTTCCCGAGCGGCTGGTGAAGCTTCCGCAGAA
GGCTCAGGTCGCCTGGCGCGAGGCAGGCGACGCCAGCTCCGACTTTGCGGTGGTGCTGCTGCATGGCATCAGCTCCGGGG
CAGCATCCTGGCTGGATGTGGCGCTGCAGCTGGCTGACAAAGTGCGCGTTCTGGCCTGGGATGCACCTGGCTATGGCGTC
TCGACGCCGCTGGCCCAATCGGCGCCTGCCGATGCCGACTATGCACAGGCGCTGGCTCAGTCGCTGCTGGTGCTGGGCGT
TCGGCGTTGCATCCTGGTCGGGCACTCGCTGGGCGCCCTGATGGCAGCCAGGCTGGCTGCCATGGCCGCGCCGGGACTGG
TCGAGCAACTGGTGCTCATCAGCCCGGCGGGCGGCTACGGCGCGACGGCCAAGGCCGAGCAGCAGGCCAAGGTGCGCGAG
GGTCGTCTGGCTTCGCTGGCCGAGAAGGGGGTCGCGGGTCTGGCGGCCGTCATCGATCAGCGCCTGGTGTCCTCCGAGGC
TCCTGAAGCCGTGCGTGCATGGGTGCGCTGGAATACGGCACGCATGCAGCCGCAGGGCTATGCGCAGGCGGTCGAGCTGC
TATGCGGCAGCGATCTGGCACAGGCACAGGGCAGGCTGGGCATGCCGGTCGAGGTTTGGGTCGGCGAGCACGATGTGGTG
ACCACACCTGCAGCCTGCAAGTCCTGGTCCGAACTGCTGGGCGCGCACTACGGTACGCTTGCGGCTGCCGGTCATGCTTC
ACCCGTGGAACAACCAATGGAGGTCGCGCATAGGCTGGCATCTTTGTTGAACCAAAGCTATTGCCCCAATACCAGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9331.64 Da        Isoelectric Point: 4.6509

>AT188414 WP_001223213.1 NZ_CP068818:c4628028-4627777 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT188414 NZ_CP090445:c5614349-5613543 [Comamonas testosteroni]
ATGAGCGAAACCCCTGAGATTTCAACCAACGAACAAGATCGTTACACCGTGCCAGCCCTGGATCGAGGACTGCGCCTGCT
GGCCTGCTTTGGTCCGGAGCAGCCGGTCTGGAGTGCGCCCGAGCTGGCGCGCAGCCTCGAGCTGCCGCGCTCCACGGTGT
TTCGCATGTTGACCACGCTTGAAAACTCGGGGTATCTGCAGCGCAGCGGTACCGAATACCGGCTCGGTCTGGCCGTGCTG
CGCCTGGGCTATGACTATCTGTCCACCCAGCCGCTGGCGCAACTGGCCGAGCCTGTGCTGCAGGCCTTGTGCGATGAGCT
GGGCATGACCAGCAATCTTGCATTGCGCGACGGCACTTCCGTGGTCTATGTGGCACGCGTCACTCCGTCCGGGGCATTCC
AGGGCGCGGTGCGCGTGGGCTCGCGTCTGCCGGCGCATGCGACGGTGCTGGGGCGGGCCCTGCTGCATGACATGGATGGC
GCGCAGCTGCGCTCGGTGTTTGGCGGCGAGGAACTGCCCCAGTTCTCCGAGAGCACGCCGCGCAACGTCGACGAGCTGCT
GCGCCTGCTGGCCGAGGATCGCGAGCGCGGCTATGCCATGGGCGAGGGCTTTTACGAGCCTGGCGTTTCCAGTATTGCAG
CGCCGGTGCGCGCCGCAGACGGGCGGGTGGTTGCGGGGCTGGCCATGGCCATTCCGTTTACCGAGTTGGGGCCCGAAAAA
ACCCAGCTCTGGGTGCAGAAGGTGCAGCAGGCGGCAGAGACTTTGTCCGCTCATTTGCGCCAGCAGCACCCGGACCATTT
GCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NWK6

Antitoxin

Source ID Structure
AlphaFold DB L4JJX7

References