Detailed information of TA system TA188405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2690180..2690818
• Replicon: chromosome
• Accession: NZ_CP068818
• Organism: Escherichia coli strain RIVM_C028803

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: JNN48_RS13085
• Protein ID: WP_000813795.1
• Coordinates: 2690642..2690818 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JNN48_RS13080
• Protein ID: WP_076797675.1
• Coordinates: 2690180..2690596 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN48_RS13060	2685332..2686273	-	942	WP_001251315.1	ABC transporter permease	-
JNN48_RS13065	2686274..2687287	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
JNN48_RS13070	2687305..2688450	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
JNN48_RS13075	2688695..2690101	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
JNN48_RS13080	2690180..2690596	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JNN48_RS13085	2690642..2690818	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JNN48_RS13090	2691040..2691270	+	231	WP_023910283.1	YncJ family protein	-
JNN48_RS13095	2691362..2693323	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JNN48_RS13100	2693396..2693932	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
JNN48_RS13105	2693985..2695196	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T188405 WP_000813795.1 NZ_CP068818:c2690818-2690642 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T188405 NZ_CP090433:c84563-84093 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT188405 WP_076797675.1 NZ_CP068818:c2690596-2690180 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT188405 NZ_CP090433:c85003-84560 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1