Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1984774..1985605 Replicon chromosome
Accession NZ_CP068818
Organism Escherichia coli strain RIVM_C028803

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag JNN48_RS09520 Protein ID WP_000854814.1
Coordinates 1984774..1985148 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag JNN48_RS09525 Protein ID WP_001285585.1
Coordinates 1985237..1985605 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN48_RS09480 1980170..1981336 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
JNN48_RS09485 1981455..1981928 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
JNN48_RS09490 1982126..1983184 + 1059 WP_001200891.1 FUSC family protein -
JNN48_RS09495 1983356..1983685 + 330 WP_000450409.1 DUF496 family protein -
JNN48_RS09500 1983786..1983968 - 183 WP_001016348.1 hypothetical protein -
JNN48_RS09505 1984040..1984168 + 129 Protein_1860 transposase domain-containing protein -
JNN48_RS09510 1984457..1984570 - 114 WP_001161660.1 DUF957 domain-containing protein -
JNN48_RS09515 1984583..1984777 - 195 WP_000988600.1 hypothetical protein -
JNN48_RS09520 1984774..1985148 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
JNN48_RS09525 1985237..1985605 - 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
JNN48_RS09530 1985679..1985900 - 222 WP_000692323.1 DUF987 domain-containing protein -
JNN48_RS09535 1985963..1986439 - 477 WP_039267628.1 RadC family protein -
JNN48_RS09540 1986455..1986934 - 480 WP_000860087.1 antirestriction protein -
JNN48_RS09545 1987016..1987834 - 819 WP_060626401.1 DUF945 domain-containing protein -
JNN48_RS09550 1987934..1988167 - 234 WP_001119729.1 DUF905 family protein -
JNN48_RS09555 1988242..1988697 - 456 WP_000581504.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T188395 WP_000854814.1 NZ_CP068818:c1985148-1984774 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T188395 NZ_CP090432:2012041-2012143 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT188395 WP_001285585.1 NZ_CP068818:c1985605-1985237 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT188395 NZ_CP090432:c2012150-2012005 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References