Detailed information of TA system TA188385

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4435401..4436236
• Replicon: chromosome
• Accession: NZ_CP068817
• Organism: Escherichia coli strain RIVM_C028786

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: JNN59_RS21335
• Protein ID: WP_001094426.1
• Coordinates: 4435401..4435778 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: JNN59_RS21340
• Protein ID: WP_001285575.1
• Coordinates: 4435868..4436236 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN59_RS21310	4430904..4431083	+	180	WP_001298063.1	hypothetical protein	-
JNN59_RS21315	4431482..4433518	-	2037	WP_000417001.1	hypothetical protein	-
JNN59_RS21320	4434470..4434628	-	159	WP_001467148.1	hypothetical protein	-
JNN59_RS21325	4434707..4434904	-	198	WP_000839272.1	DUF957 domain-containing protein	-
JNN59_RS21330	4434916..4435404	-	489	WP_000761683.1	hypothetical protein	-
JNN59_RS21335	4435401..4435778	-	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
JNN59_RS21340	4435868..4436236	-	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN59_RS21345	4436316..4436537	-	222	WP_000692350.1	DUF987 domain-containing protein	-
JNN59_RS21350	4436624..4437100	-	477	WP_001298074.1	RadC family protein	-
JNN59_RS21355	4437115..4437600	-	486	WP_000213697.1	antirestriction protein	-
JNN59_RS21360	4437691..4438509	-	819	WP_001175160.1	DUF945 domain-containing protein	-
JNN59_RS21365	4438599..4438832	-	234	WP_001278290.1	DUF905 family protein	-
JNN59_RS21370	4438838..4439515	-	678	WP_001097301.1	hypothetical protein	-
JNN59_RS21375	4439663..4440343	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T188385 WP_001094426.1 NZ_CP068817:c4435778-4435401 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T188385 NZ_CP090429:c4649513-4648980 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT188385 WP_001285575.1 NZ_CP068817:c4436236-4435868 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188385 NZ_CP090429:c4649790-4649524 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAGTGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5