Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4235093..4235643 Replicon chromosome
Accession NZ_CP068817
Organism Escherichia coli strain RIVM_C028786

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag JNN59_RS20310 Protein ID WP_001160966.1
Coordinates 4235093..4235407 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag JNN59_RS20315 Protein ID WP_000125568.1
Coordinates 4235410..4235643 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN59_RS20285 4230768..4232054 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
JNN59_RS20290 4232054..4233043 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
JNN59_RS20295 4233040..4233861 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
JNN59_RS20300 4233864..4234241 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
JNN59_RS20305 4234248..4235096 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
JNN59_RS20310 4235093..4235407 - 315 WP_001160966.1 CcdB family protein Toxin
JNN59_RS20315 4235410..4235643 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
JNN59_RS20320 4235729..4236208 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
JNN59_RS20325 4236400..4238262 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
JNN59_RS20330 4238255..4238785 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
JNN59_RS20335 4238893..4240224 - 1332 WP_001183198.1 MFS transporter -
JNN59_RS20340 4240283..4240570 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T188378 WP_001160966.1 NZ_CP068817:c4235407-4235093 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T188378 NZ_CP090428:c919207-918923 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT188378 WP_000125568.1 NZ_CP068817:c4235643-4235410 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT188378 NZ_CP090428:c918926-918633 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References