Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 3220150..3221870 Replicon chromosome
Accession NZ_CP068817
Organism Escherichia coli strain RIVM_C028786

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag JNN59_RS15440 Protein ID WP_000312833.1
Coordinates 3220150..3220806 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag JNN59_RS15445 Protein ID WP_000634203.1
Coordinates 3220803..3221870 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN59_RS15420 3215550..3215804 - 255 WP_154670580.1 hypothetical protein -
JNN59_RS15425 3216086..3216325 + 240 WP_001431807.1 hypothetical protein -
JNN59_RS15430 3216365..3218803 + 2439 WP_000985465.1 type I restriction-modification system subunit M -
JNN59_RS15435 3218803..3220137 + 1335 WP_000228013.1 restriction endonuclease subunit S -
JNN59_RS15440 3220150..3220806 + 657 WP_000312833.1 DUF4433 domain-containing protein Toxin
JNN59_RS15445 3220803..3221870 + 1068 WP_000634203.1 macro domain-containing protein Antitoxin
JNN59_RS15450 3221889..3224984 + 3096 WP_000108735.1 HsdR family type I site-specific deoxyribonuclease -
JNN59_RS15455 3224984..3225700 + 717 WP_001122107.1 M48 family metallopeptidase -
JNN59_RS15460 3226045..3226659 + 615 Protein_3036 IS3-like element IS600 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - aafC / aggD / iroC / iroD / iroE / iroN 3214143..3274715 60572


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T188373 WP_000312833.1 NZ_CP068817:3220150-3220806 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

>T188373 NZ_CP090418:c109161-107839 [Stenotrophomonas maltophilia]
ATGGGTACGCTGCGGGTCTGGATGAATGGTGTCGAGGTCGCGCTCTGGGATGATGTCCGGAGCGGCTCTTCCCGGCTTTC
GTACGTCGACTCGTGGATCAGCTCCGCCCAGTCCCGGCCGTTGTCGCTCTCACTGCCCCTGCTCCCGAAGGGTGAGAGCC
ATCGGGGCAATGTCGTCAACAACTACTTCGACAACCTTCTTCCCGACAACGCCGTCATCCGCAACCGGATCCGCGACAGG
TTCTCCACGCGCAGCAGCGACACATTCGATCTTCTCGAAGCCATTGGCCGGGATTGCGTCGGCGCAGTGCAACTGCTGCC
GCTTTCGCATACACCGGGGGACATCCAGCAGATTACGTGCGAAGCGTTGGATGAATCAGGCGTCGCTGCGATCCTGCGTC
AGGTGACGGCAGGGAATCTACCTGGCGCACCCACCAGTGATGATGCGTTCCGCATATCCATCGCCGGTGCGCAGGAGAAG
ACGGGACTTCTGCTGCATGCTGGCCAGTGGTGCATTCCTACCGGCAGTACCCCATCCACCCATATCTTCAAGCTGCCGCT
TGGCATTGTGGGCAACATGCGGGCGGACATGCACCAATCCGTCGAGAACGAATGGCTGTGCATGCAACTGCTTTCCGCCT
TTGGAATCCCCGTTGCGAATACGCAGATGGGACGCTTCAAGGACGAACGCGCGCTTATTGTGGAGCGATTCGACCGCCGC
CTTTCTGCGGATCGCCGGTGGTGGCTGCGGCTGCCGCAGGAAGACATGTGCCAGGTCTTCGGTCTGCCCGCTGCCAGGAA
GTATGAATCCGATGGCGGCCCAGGCATCGTGACCATCATGGATCTGCTTCGACAATCCGAGCATTTCGAGGATCGGGCGA
CCTTCTTCAAGACGCAGATTCTTTTCTGGATGTTGGCTGCAACCGATGGACATGCCAAGAACTTCAGTGTCTACCTCGAA
GCGGATGGACGCTTCCGCCTGACGCCCGTCTACGACGTATTGTCGGTGCATCCCATCCTGGGTAGGGGGCCAAACCAGCT
CGACCCGCAGAATGCACAGCTCGCCATGTCCGTCCAGGGCAAGAACCGCCACCGGAAGCTGCACCTTGTGCGACGACGCC
ACTGGAACGAAACAGCACGCGCCTGTGGTGTCGCTGATGGTGCGGAGCCGTGGATCGAAGCGCTGATTGCAGCCGTCGAA
CCCGCCATCGAGCAGGTCACGCGTCAGCTCCCAAGTGATTTCCCCGTAAACGTGTCCGAACCCATTTTCAACGGGCTGCG
CGCTGCAGCGGCACGCCTTTCCAGCATGTCGCCGGACGCCTGA

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT188373 WP_000634203.1 NZ_CP068817:3220803-3221870 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp

>AT188373 NZ_CP090418:c109422-109165 [Stenotrophomonas maltophilia]
ATGGACAGGCCGCTGCACACGGTGAGGACACCTCAACAACTGGCACCGCTGATGCGTGCGTTCAGGCGCCAGGCGGGCCT
GAGCCAGGCGCAGTTGGCGGCACAGCTCGGCATAAGCCGCCAGGCAGTAGGCGCATTGGAGCGCGACCCGGCATCGGCAA
GCTTCGAACGGCTGATGCGGGTCTGGGCAGTGCTGGGTCTGGAAGTCAGTTTGCAGCAGCGAAGTCCCCCGGAGACAACG
CCAACGTTGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References