Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2567182..2568770 Replicon chromosome
Accession NZ_CP068817
Organism Escherichia coli strain RIVM_C028786

Toxin (Protein)


Gene name hipA Uniprot ID A0A192ENQ5
Locus tag JNN59_RS12120 Protein ID WP_001125432.1
Coordinates 2567448..2568770 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag JNN59_RS12115 Protein ID WP_001304368.1
Coordinates 2567182..2567448 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN59_RS12100 2563550..2565001 + 1452 WP_000854642.1 tagaturonate reductase -
JNN59_RS12105 2565198..2566112 + 915 WP_001298637.1 bestrophin family protein -
JNN59_RS12110 2566116..2566874 - 759 WP_001286538.1 trans-aconitate 2-methyltransferase -
JNN59_RS12115 2567182..2567448 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
JNN59_RS12120 2567448..2568770 + 1323 WP_001125432.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
JNN59_RS12125 2568946..2569638 - 693 WP_000382466.1 hypothetical protein -
JNN59_RS12130 2569650..2569925 - 276 WP_000948310.1 hypothetical protein -
JNN59_RS12135 2569927..2570067 - 141 WP_001335097.1 hypothetical protein -
JNN59_RS12140 2570183..2570365 - 183 WP_001335098.1 hypothetical protein -
JNN59_RS12145 2570535..2571587 + 1053 WP_000568119.1 hypothetical protein -
JNN59_RS12150 2571599..2571916 + 318 WP_000073834.1 hypothetical protein -
JNN59_RS12155 2572120..2572362 + 243 WP_000699011.1 phage capsid protein -
JNN59_RS12160 2572409..2573629 + 1221 WP_033546003.1 attachment protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49297.02 Da        Isoelectric Point: 8.6972

>T188370 WP_001125432.1 NZ_CP068817:2567448-2568770 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

>T188370 NZ_CP090414:c916029-915775 [Bifidobacterium longum]
ATGCTGCTCTGCTGGACCGATGACGCCTGGGCCGACTACCTGTACTGGCAGGCTCAGGACCGCAGGACCCTCAAGCGCGT
CAACACGCTCATCCGGGATATGCAGCGTACCCCGTTCGAGGGCATCGGCAAGCCCGAACCCCTCAAATGGGGCCTGTCCG
GCGCATGGTCGCGGCGCATCGACTCAGCCAACCGTATCATCTATACCGTCGCCGACGACAGGCTCTGCATCCTCTCCGCG
AAGGACCACTACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT188370 WP_001304368.1 NZ_CP068817:2567182-2567448 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT188370 NZ_CP090414:c916295-916029 [Bifidobacterium longum]
ATGACACAGGCCGTGGCCTACAGCAATTTCAGGGCGAATCTCAAGACCTATATGCGCAGGGTCAACGAGGACGCCGACAC
CCTTCTCGTGACCAACGCGGACCCGGAGGACAACGTGGTCGTGATGAGCGCCGACGACTACGATTCGCTCATGGAGACGC
TGCGCGTCTACCAGAACCCGTATCTGAGCGATAAGGTGATGCGCGGCATGGCGCAGGTACGGCAGGGCCAGACGATGGCC
CATGACCTTGTCGAAGCGGGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192ENQ5

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References