Detailed information of TA system TA188361

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1995825..1996656
• Replicon: chromosome
• Accession: NZ_CP068817
• Organism: Escherichia coli strain RIVM_C028786

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JNN59_RS09410
• Protein ID: WP_000854814.1
• Coordinates: 1995825..1996199 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN59_RS09415
• Protein ID: WP_024174331.1
• Coordinates: 1996288..1996656 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN59_RS09370	1991221..1992387	+	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
JNN59_RS09375	1992506..1992979	+	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
JNN59_RS09380	1993177..1994235	+	1059	WP_001200890.1	FUSC family protein	-
JNN59_RS09385	1994407..1994736	+	330	WP_000450409.1	DUF496 family protein	-
JNN59_RS09390	1994837..1995019	-	183	WP_001016348.1	hypothetical protein	-
JNN59_RS09395	1995073..1995219	+	147	Protein_1840	transposase domain-containing protein	-
JNN59_RS09400	1995508..1995621	-	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN59_RS09405	1995634..1995828	-	195	WP_000988601.1	hypothetical protein	-
JNN59_RS09410	1995825..1996199	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN59_RS09415	1996288..1996656	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN59_RS09420	1996730..1996951	-	222	WP_000692346.1	DUF987 domain-containing protein	-
JNN59_RS09425	1997020..1997496	-	477	WP_001541535.1	RadC family protein	-
JNN59_RS09430	1997512..1997985	-	474	WP_000855074.1	antirestriction protein	-
JNN59_RS09435	1998248..1999069	-	822	WP_001234571.1	DUF945 domain-containing protein	-
JNN59_RS09440	1999290..1999700	-	411	WP_000846704.1	hypothetical protein	-
JNN59_RS09445	1999716..2000393	-	678	WP_001362823.1	hypothetical protein	-
JNN59_RS09450	2000529..2001599	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T188361 WP_000854814.1 NZ_CP068817:c1996199-1995825 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T188361 NZ_CP090405:44459-44713 [Shigella sp. PIB]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT188361 WP_024174331.1 NZ_CP068817:c1996656-1996288 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT188361 NZ_CP090405:44710-44952 [Shigella sp. PIB]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A829FY50