Detailed information of TA system TA188357

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 830483..831315
• Replicon: chromosome
• Accession: NZ_CP068817
• Organism: Escherichia coli strain RIVM_C028786

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: JNN59_RS03970
• Protein ID: WP_000854753.1
• Coordinates: 830483..830857 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN59_RS03975
• Protein ID: WP_001531802.1
• Coordinates: 830947..831315 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN59_RS03940	825598..826746	-	1149	WP_000905914.1	capsule polysaccharide transporter	-
JNN59_RS03945	826818..827801	-	984	WP_001335608.1	KpsF/GutQ family sugar-phosphate isomerase	-
JNN59_RS03950	828611..828781	-	171	Protein_775	IS110 family transposase	-
JNN59_RS03955	829123..829692	-	570	WP_001290247.1	DUF4942 domain-containing protein	-
JNN59_RS03960	829789..829986	-	198	WP_000839281.1	DUF957 domain-containing protein	-
JNN59_RS03965	829998..830486	-	489	WP_000777541.1	hypothetical protein	-
JNN59_RS03970	830483..830857	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
JNN59_RS03975	830947..831315	-	369	WP_001531802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN59_RS03980	831478..831699	-	222	WP_000692329.1	DUF987 domain-containing protein	-
JNN59_RS03985	831762..832238	-	477	WP_001186726.1	RadC family protein	-
JNN59_RS03990	832254..832739	-	486	WP_001531954.1	antirestriction protein	-
JNN59_RS03995	832794..833615	-	822	WP_202847156.1	DUF945 domain-containing protein	-
JNN59_RS04000	833696..833907	+	212	Protein_785	hypothetical protein	-
JNN59_RS04005	834026..834481	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	828611..828715	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T188357 WP_000854753.1 NZ_CP068817:c830857-830483 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T188357 NZ_CP090403:c62603-62298 [Shigella sp. PIB]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13422.27 Da
• Isoelectric Point: 6.3161

>AT188357 WP_001531802.1 NZ_CP068817:c831315-830947 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNSLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188357 NZ_CP090403:c62823-62605 [Shigella sp. PIB]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0