Detailed information of TA system TA188342

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3861594..3862429
• Replicon: chromosome
• Accession: NZ_CP068816
• Organism: Escherichia coli strain RIVM_C028724

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q83W73
• Locus tag: JNN36_RS18400
• Protein ID: WP_000854700.1
• Coordinates: 3861594..3861971 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN36_RS18405
• Protein ID: WP_001531802.1
• Coordinates: 3862061..3862429 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN36_RS18370	3856916..3857410	+	495	WP_001059463.1	MarR family transcriptional regulator	-
JNN36_RS18375	3857845..3858183	-	339	WP_000772639.1	Arm DNA-binding domain-containing protein	-
JNN36_RS18380	3858549..3859868	+	1320	WP_000144686.1	site-specific integrase	-
JNN36_RS18385	3859961..3860815	-	855	WP_024174313.1	DUF4942 domain-containing protein	-
JNN36_RS18390	3860900..3861097	-	198	WP_000839247.1	DUF957 domain-containing protein	-
JNN36_RS18395	3861109..3861597	-	489	WP_000761726.1	hypothetical protein	-
JNN36_RS18400	3861594..3861971	-	378	WP_000854700.1	TA system toxin CbtA family protein	Toxin
JNN36_RS18405	3862061..3862429	-	369	WP_001531802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN36_RS18410	3862592..3862813	-	222	WP_000692353.1	DUF987 domain-containing protein	-
JNN36_RS18415	3862882..3863358	-	477	WP_001186199.1	RadC family protein	-
JNN36_RS18420	3863374..3863859	-	486	WP_000213723.1	antirestriction protein	-
JNN36_RS18425	3863951..3864769	-	819	WP_001234709.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.28 Da
• Isoelectric Point: 7.8046

>T188342 WP_000854700.1 NZ_CP068816:c3861971-3861594 [Escherichia coli]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T188342 NZ_CP090402:1887104-1887358 [Shigella sp. PIB]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13422.27 Da
• Isoelectric Point: 6.3161

>AT188342 WP_001531802.1 NZ_CP068816:c3862429-3862061 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNSLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188342 NZ_CP090402:1886856-1887107 [Shigella sp. PIB]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A454A1I2