Detailed information of TA system TA188339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3180022..3181742
• Replicon: chromosome
• Accession: NZ_CP068816
• Organism: Escherichia coli strain RIVM_C028724

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A7U9J2A7
• Locus tag: JNN36_RS15145
• Protein ID: WP_000312833.1
• Coordinates: 3180022..3180678 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: B7UP19
• Locus tag: JNN36_RS15150
• Protein ID: WP_000634203.1
• Coordinates: 3180675..3181742 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN36_RS15125	3175422..3175676	-	255	WP_154670580.1	hypothetical protein	-
JNN36_RS15130	3175958..3176197	+	240	WP_001431807.1	hypothetical protein	-
JNN36_RS15135	3176237..3178675	+	2439	WP_000985465.1	type I restriction-modification system subunit M	-
JNN36_RS15140	3178675..3180009	+	1335	WP_000228013.1	restriction endonuclease subunit S	-
JNN36_RS15145	3180022..3180678	+	657	WP_000312833.1	DUF4433 domain-containing protein	Toxin
JNN36_RS15150	3180675..3181742	+	1068	WP_000634203.1	macro domain-containing protein	Antitoxin
JNN36_RS15155	3181761..3184856	+	3096	WP_000108735.1	HsdR family type I site-specific deoxyribonuclease	-
JNN36_RS15160	3184856..3185572	+	717	WP_001122107.1	M48 family metallopeptidase	-
JNN36_RS15165	3185917..3186531	+	615	Protein_2978	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	aafC / aggD / iroC / iroD / iroE / iroN	3174015..3233809	59794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25233.56 Da
• Isoelectric Point: 6.2145

>T188339 WP_000312833.1 NZ_CP068816:3180022-3180678 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T188339 NZ_CP090402:910820-911227 [Shigella sp. PIB]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40672.70 Da
• Isoelectric Point: 5.6927

>AT188339 WP_000634203.1 NZ_CP068816:3180675-3181742 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT188339 NZ_CP090402:910573-910839 [Shigella sp. PIB]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9J2A7

Antitoxin

• AlphaFold DB: A0A7U9J2B9