Detailed information of TA system TA188338

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3162400..3163244
• Replicon: chromosome
• Accession: NZ_CP068816
• Organism: Escherichia coli strain RIVM_C028724

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: JNN36_RS15060
• Protein ID: WP_000854686.1
• Coordinates: 3162400..3162783 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: JNN36_RS15065
• Protein ID: WP_001285602.1
• Coordinates: 3162864..3163244 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN36_RS15030	3159076..3159780	+	705	WP_001241673.1	leucyl/phenylalanyl-tRNA--protein transferase	-
JNN36_RS15035	3160065..3160283	+	219	WP_001040187.1	translation initiation factor IF-1	-
JNN36_RS15045	3160774..3161616	-	843	WP_001431817.1	DUF4942 domain-containing protein	-
JNN36_RS15050	3161701..3161898	-	198	WP_000839253.1	DUF957 domain-containing protein	-
JNN36_RS15055	3161915..3162403	-	489	WP_001054233.1	hypothetical protein	-
JNN36_RS15060	3162400..3162783	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
JNN36_RS15065	3162864..3163244	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN36_RS15070	3163255..3163938	-	684	WP_000086768.1	hypothetical protein	-
JNN36_RS15075	3163957..3164178	-	222	WP_000692298.1	DUF987 domain-containing protein	-
JNN36_RS15080	3164241..3164717	-	477	WP_001186726.1	RadC family protein	-
JNN36_RS15085	3164733..3165218	-	486	WP_000214307.1	antirestriction protein	-
JNN36_RS15090	3165310..3166128	-	819	WP_001234732.1	DUF945 domain-containing protein	-
JNN36_RS15095	3166228..3166461	-	234	WP_001119719.1	DUF905 family protein	-
JNN36_RS15100	3166540..3166995	-	456	WP_001504120.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T188338 WP_000854686.1 NZ_CP068816:c3162783-3162400 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T188338 NZ_CP090402:c634958-634494 [Shigella sp. PIB]
ATGGATTTTCCACAAAGGGTTAATAGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT188338 WP_001285602.1 NZ_CP068816:c3163244-3162864 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT188338 NZ_CP090402:c635293-634958 [Shigella sp. PIB]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA