Detailed information of TA system TA188336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2530950..2532538
• Replicon: chromosome
• Accession: NZ_CP068816
• Organism: Escherichia coli strain RIVM_C028724

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A192ENQ5
• Locus tag: JNN36_RS11890
• Protein ID: WP_001125432.1
• Coordinates: 2531216..2532538 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: JNN36_RS11885
• Protein ID: WP_001304368.1
• Coordinates: 2530950..2531216 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN36_RS11870	2527318..2528769	+	1452	WP_000854642.1	tagaturonate reductase	-
JNN36_RS11875	2528966..2529880	+	915	WP_001298637.1	bestrophin family protein	-
JNN36_RS11880	2529884..2530642	-	759	WP_001286538.1	trans-aconitate 2-methyltransferase	-
JNN36_RS11885	2530950..2531216	+	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JNN36_RS11890	2531216..2532538	+	1323	WP_001125432.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JNN36_RS11895	2532714..2533406	-	693	WP_000382466.1	hypothetical protein	-
JNN36_RS11900	2533418..2533693	-	276	WP_000948310.1	hypothetical protein	-
JNN36_RS11905	2533695..2533835	-	141	WP_001335097.1	hypothetical protein	-
JNN36_RS11910	2533951..2534133	-	183	WP_001335098.1	hypothetical protein	-
JNN36_RS11915	2534303..2535355	+	1053	WP_000568119.1	hypothetical protein	-
JNN36_RS11920	2535367..2535684	+	318	WP_000073834.1	hypothetical protein	-
JNN36_RS11925	2535888..2536130	+	243	WP_000699011.1	phage capsid protein	-
JNN36_RS11930	2536177..2537457	+	1281	WP_000823306.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2530932..2550238	19306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49297.02 Da
• Isoelectric Point: 8.6972

>T188336 WP_001125432.1 NZ_CP068816:2531216-2532538 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T188336 NZ_CP090402:158197-158382 [Shigella sp. PIB]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT188336 WP_001304368.1 NZ_CP068816:2530950-2531216 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT188336 NZ_CP090402:158399-158809 [Shigella sp. PIB]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTATGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A192ENQ5

Antitoxin

• AlphaFold DB: V0VAH2