Detailed information of TA system TA188327

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1959593..1960424
• Replicon: chromosome
• Accession: NZ_CP068816
• Organism: Escherichia coli strain RIVM_C028724

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JNN36_RS09180
• Protein ID: WP_000854814.1
• Coordinates: 1959593..1959967 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN36_RS09185
• Protein ID: WP_024174331.1
• Coordinates: 1960056..1960424 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN36_RS09140	1954989..1956155	+	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
JNN36_RS09145	1956274..1956747	+	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
JNN36_RS09150	1956945..1958003	+	1059	WP_001200890.1	FUSC family protein	-
JNN36_RS09155	1958175..1958504	+	330	WP_000450409.1	DUF496 family protein	-
JNN36_RS09160	1958605..1958787	-	183	WP_001016348.1	hypothetical protein	-
JNN36_RS09165	1958841..1958987	+	147	Protein_1795	transposase domain-containing protein	-
JNN36_RS09170	1959276..1959389	-	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN36_RS09175	1959402..1959596	-	195	WP_000988601.1	hypothetical protein	-
JNN36_RS09180	1959593..1959967	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN36_RS09185	1960056..1960424	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN36_RS09190	1960498..1960719	-	222	WP_000692346.1	DUF987 domain-containing protein	-
JNN36_RS09195	1960788..1961264	-	477	WP_001541535.1	RadC family protein	-
JNN36_RS09200	1961280..1961753	-	474	WP_000855074.1	antirestriction protein	-
JNN36_RS09205	1962016..1962837	-	822	WP_001234571.1	DUF945 domain-containing protein	-
JNN36_RS09210	1963058..1963468	-	411	WP_000846704.1	hypothetical protein	-
JNN36_RS09215	1963484..1964161	-	678	WP_001362823.1	hypothetical protein	-
JNN36_RS09220	1964297..1965367	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T188327 WP_000854814.1 NZ_CP068816:c1959967-1959593 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T188327 NZ_CP090400:871821-872111 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT188327 WP_024174331.1 NZ_CP068816:c1960424-1960056 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT188327 NZ_CP090400:871583-871831 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A829FY50