Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4676957..4677454 Replicon chromosome
Accession NZ_CP068815
Organism Escherichia coli strain RIVM_C028620

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN49_RS22380 Protein ID WP_001173343.1
Coordinates 4676957..4677130 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag JNN49_RS22385 Protein ID WP_001312331.1
Coordinates 4677158..4677454 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN49_RS22370 4673627..4675084 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
JNN49_RS22375 4675321..4676838 + 1518 WP_000003805.1 lysine--tRNA ligase -
JNN49_RS22380 4676957..4677130 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN49_RS22385 4677158..4677454 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN49_RS22390 4677682..4677954 - 273 WP_000405647.1 N-acetyltransferase -
JNN49_RS22395 4677966..4678196 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
JNN49_RS22400 4678377..4680008 + 1632 WP_001216466.1 sensor histidine kinase -
JNN49_RS22405 4680005..4680724 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T188318 WP_001173343.1 NZ_CP068815:c4677130-4676957 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T188318 NZ_CP090399:c2372264-2371326 [Vibrio cholerae]
ATGGATAAAGATAGCCCATATTACAAACAGGTTTCCTTGCTCATAAGAATGCTTCCTGTGGTAGCAACAGAGACGGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATTTTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAGATGAGGCTTTGATTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCAGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATAGTTGTATCAAGTCCGGTTGCGACGATCAA
AATTGAAGTGTCGCCCGTCGCCAGAGGTACATTGCATAGTGCAGAAATAATGCCAGTTCAAGAGTCTGTTGAAGACGAGT
TTGGTTATGCTGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGGAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTTGATGTGCGTATGTTACTTGGCAGTGAAGGGATTTCGAGAGAGATTTTGGTGGGTTTTTTAACCTA
TACATTAAGTCACCCTCGGCCTATTAATGAAGTCATGTCGCCAAACTGGCAGCCTCTGAATGAGAAATTTCAGGCAGAAT
TTGACGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCCTCTGTTAGGCCCATGATGTTAATTGAACTGCAAAAA
CATTTCACAGAAAGGGATCATGCTTTCCTTATGTCATTCAAAAGAGGGCAACCTGACTGGGCATTGTTTGACTATCCTAA
TGCAGCAGACTTGCCAGCGATTCGTTGGAAATTGCAGAACATTAACAAATTGGCAAAGAATCAAGCAAAACACCAAGAGC
AATTAGATAAATTGAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT188318 WP_001312331.1 NZ_CP068815:c4677454-4677158 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT188318 NZ_CP090399:c2373048-2372257 [Vibrio cholerae]
ATGTCATCTAAAATAAACTGGCTTGTTGCTCATACTTCTCCTGGTGCGCTAGTACTTCAGCAATGGCTGACTGAAAACGG
CGTGAGCTACTCGTTGGCTCAAAAGTACGCGCAGAACGGTTGGCTGAAGAAGCTTAGTTCTGGTGTGTACTATCGTCCAA
ATGCGCAGGGCGATATAAAGCCAACTTGGGTTGATGCCATTCAAGCATTGGATGTGCAATTGGGCGTTCCAGTTCATTTG
GCTGGATTGAGCAGCTTAACTCACCAAGGACTGAGTCACTATTTGCAGCTGAACAAAGAGCAAGTTTGGATTTGCGTTAA
AAACAAGTCGTCCTTACCGAAATGGTTTCGTGAATTCCCTTATCAAAATTGGTTTTACTGCGGAAACCATAAGCTTGAAG
TGAATCCCGAGAAAGATTTGAAAAGGATCACGGTTAAAGAGAAAGAGCTCACTGTCAGCTGTGCAGAACTTGCTGCCTAT
GAAGTGGTAGATGCGATTGGAAAGCTGATTTCATTTGAGCATGTCGCAGAATTATTTCAGGGTTTAGTTAATCTTAGCCC
TAGAAAAGTACAAGATATTCTTGAACGAAGCAGCTCTGTTCAGGCAAACCGAATATTTCTATTTCTGGGTCGATACTACG
ATCACCAGTGGGTCAATCGCGTAGATGAAACAAGAATTAAATTGGGGGCAGGAAAGCGGCAGGTTGTCGAAAAAGGACGT
TTTGATGAGCGATATCAAATCACAGTGCCAGAGATATTAAGCGTCAAAAAAGGTGAACAACATAATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References