Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4207039..4207589 Replicon chromosome
Accession NZ_CP068815
Organism Escherichia coli strain RIVM_C028620

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag JNN49_RS20095 Protein ID WP_001160966.1
Coordinates 4207039..4207353 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag JNN49_RS20100 Protein ID WP_000125568.1
Coordinates 4207356..4207589 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN49_RS20070 4202714..4204000 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
JNN49_RS20075 4204000..4204989 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
JNN49_RS20080 4204986..4205807 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
JNN49_RS20085 4205810..4206187 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
JNN49_RS20090 4206194..4207042 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
JNN49_RS20095 4207039..4207353 - 315 WP_001160966.1 CcdB family protein Toxin
JNN49_RS20100 4207356..4207589 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
JNN49_RS20105 4207675..4208154 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
JNN49_RS20110 4208346..4210208 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
JNN49_RS20115 4210201..4210731 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
JNN49_RS20120 4210839..4212170 - 1332 WP_001183198.1 MFS transporter -
JNN49_RS20125 4212229..4212516 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T188310 WP_001160966.1 NZ_CP068815:c4207353-4207039 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T188310 NZ_CP090398:881308-881715 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT188310 WP_000125568.1 NZ_CP068815:c4207589-4207356 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT188310 NZ_CP090398:881061-881327 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References