Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3870691..3871526 Replicon chromosome
Accession NZ_CP068815
Organism Escherichia coli strain RIVM_C028620

Toxin (Protein)


Gene name yeeV Uniprot ID Q83W73
Locus tag JNN49_RS18470 Protein ID WP_000854700.1
Coordinates 3870691..3871068 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID E3PJ72
Locus tag JNN49_RS18475 Protein ID WP_001278232.1
Coordinates 3871158..3871526 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN49_RS18440 3866013..3866507 + 495 WP_001059463.1 MarR family transcriptional regulator -
JNN49_RS18445 3866942..3867280 - 339 WP_000772639.1 Arm DNA-binding domain-containing protein -
JNN49_RS18450 3867646..3868965 + 1320 WP_000144686.1 site-specific integrase -
JNN49_RS18455 3869058..3869912 - 855 WP_024174313.1 DUF4942 domain-containing protein -
JNN49_RS18460 3869997..3870194 - 198 WP_000839247.1 DUF957 domain-containing protein -
JNN49_RS18465 3870206..3870694 - 489 WP_000761726.1 hypothetical protein -
JNN49_RS18470 3870691..3871068 - 378 WP_000854700.1 TA system toxin CbtA family protein Toxin
JNN49_RS18475 3871158..3871526 - 369 WP_001278232.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
JNN49_RS18480 3871689..3871910 - 222 WP_000692353.1 DUF987 domain-containing protein -
JNN49_RS18485 3871979..3872455 - 477 WP_001186199.1 RadC family protein -
JNN49_RS18490 3872471..3872956 - 486 WP_000213723.1 antirestriction protein -
JNN49_RS18495 3873048..3873866 - 819 WP_001234709.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / vat 3822755..3899428 76673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14276.28 Da        Isoelectric Point: 7.8046

>T188308 WP_000854700.1 NZ_CP068815:c3871068-3870691 [Escherichia coli]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T188308 NZ_CP090398:356244-356591 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13491.37 Da        Isoelectric Point: 6.6290

>AT188308 WP_001278232.1 NZ_CP068815:c3871526-3871158 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT188308 NZ_CP090398:356591-356890 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A1I2

Antitoxin

Source ID Structure
AlphaFold DB E3PJ72

References