Detailed information of TA system TA188302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2531740..2533328
• Replicon: chromosome
• Accession: NZ_CP068815
• Organism: Escherichia coli strain RIVM_C028620

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A192ENQ5
• Locus tag: JNN49_RS11895
• Protein ID: WP_001125432.1
• Coordinates: 2532006..2533328 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: JNN49_RS11890
• Protein ID: WP_001304368.1
• Coordinates: 2531740..2532006 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN49_RS11875	2528108..2529559	+	1452	WP_000854642.1	tagaturonate reductase	-
JNN49_RS11880	2529756..2530670	+	915	WP_001298637.1	bestrophin family protein	-
JNN49_RS11885	2530674..2531432	-	759	WP_001286538.1	trans-aconitate 2-methyltransferase	-
JNN49_RS11890	2531740..2532006	+	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JNN49_RS11895	2532006..2533328	+	1323	WP_001125432.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JNN49_RS11900	2533504..2534196	-	693	WP_000382466.1	hypothetical protein	-
JNN49_RS11905	2534208..2534483	-	276	WP_000948310.1	hypothetical protein	-
JNN49_RS11910	2534485..2534625	-	141	WP_001335097.1	hypothetical protein	-
JNN49_RS11915	2534741..2534923	-	183	WP_001335098.1	hypothetical protein	-
JNN49_RS11920	2535093..2536145	+	1053	WP_000568119.1	hypothetical protein	-
JNN49_RS11925	2536157..2536474	+	318	WP_000073834.1	hypothetical protein	-
JNN49_RS11930	2536678..2536920	+	243	WP_000699011.1	phage capsid protein	-
JNN49_RS11935	2536967..2538247	+	1281	WP_000823306.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49297.02 Da
• Isoelectric Point: 8.6972

>T188302 WP_001125432.1 NZ_CP068815:2532006-2533328 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T188302 NZ_CP090397:3971473-3971691 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT188302 WP_001304368.1 NZ_CP068815:2531740-2532006 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT188302 NZ_CP090397:3971072-3971446 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A192ENQ5

Antitoxin

• AlphaFold DB: V0VAH2