Detailed information of TA system TA188289

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 830483..831315
• Replicon: chromosome
• Accession: NZ_CP068815
• Organism: Escherichia coli strain RIVM_C028620

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: JNN49_RS03965
• Protein ID: WP_000854753.1
• Coordinates: 830483..830857 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: JNN49_RS03970
• Protein ID: WP_001278232.1
• Coordinates: 830947..831315 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN49_RS03935	825598..826746	-	1149	WP_000905914.1	capsule polysaccharide transporter	-
JNN49_RS03940	826818..827801	-	984	WP_001335608.1	KpsF/GutQ family sugar-phosphate isomerase	-
JNN49_RS03945	828611..828781	-	171	Protein_775	IS110 family transposase	-
JNN49_RS03950	829123..829692	-	570	WP_001290247.1	DUF4942 domain-containing protein	-
JNN49_RS03955	829789..829986	-	198	WP_000839281.1	DUF957 domain-containing protein	-
JNN49_RS03960	829998..830486	-	489	WP_000777541.1	hypothetical protein	-
JNN49_RS03965	830483..830857	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
JNN49_RS03970	830947..831315	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
JNN49_RS03975	831478..831699	-	222	WP_000692329.1	DUF987 domain-containing protein	-
JNN49_RS03980	831762..832238	-	477	WP_001186726.1	RadC family protein	-
JNN49_RS03985	832254..832739	-	486	WP_001531954.1	antirestriction protein	-
JNN49_RS03990	832794..833615	-	822	WP_202847156.1	DUF945 domain-containing protein	-
JNN49_RS03995	833696..833907	+	212	Protein_785	hypothetical protein	-
JNN49_RS04000	834026..834481	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	828611..828715	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T188289 WP_000854753.1 NZ_CP068815:c830857-830483 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T188289 NZ_CP090393:c4637559-4637227 [Klebsiella pneumoniae]
ATGAAAACTTTACCTGCAACAACTCCGCAGACGGCGAAGCTATGTCTGACGTCCGCGGATACCTGGCAAATGTTACTGAC
ACGTCTGCTAGAACAGCATTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCAGGGATCCCTCTGGCCGATGCAGTTAACTTTCTGGTGGATAAGTACGCACTGGTTCGTATCGATCGCAGAGGATTG
AGTTGGCAGGAGCAATCATCCTATCTCCGGCTTGTCGATACTCAGCGAACCATTAAGGTTATTGAGTTGTGGCTATTTGG
TGTAATGATGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT188289 WP_001278232.1 NZ_CP068815:c831315-830947 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188289 NZ_CP090393:c4637897-4637580 [Klebsiella pneumoniae]
ATGAGCAACATCACTTGGGGCCTGCAGCGGGATATCACGCCGTGCCTGGGATCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGAGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGTTGACCACTGGTGAAATGAACCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTCCGGCTACGTATACATCGCTGTTTATCTCACCCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: E3PJ72