Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4870197..4870694 Replicon chromosome
Accession NZ_CP068814
Organism Escherichia coli strain RIVM_C028613

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN56_RS23540 Protein ID WP_001173343.1
Coordinates 4870197..4870370 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN56_RS23545 Protein ID WP_000398619.1
Coordinates 4870398..4870694 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN56_RS23530 4866867..4868324 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
JNN56_RS23535 4868561..4870078 + 1518 WP_001550472.1 lysine--tRNA ligase -
JNN56_RS23540 4870197..4870370 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN56_RS23545 4870398..4870694 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN56_RS23550 4870923..4871195 - 273 WP_000405647.1 N-acetyltransferase -
JNN56_RS23555 4871207..4871437 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
JNN56_RS23560 4871617..4873248 + 1632 WP_001216477.1 sensor histidine kinase -
JNN56_RS23565 4873245..4873964 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4839780..4870694 30914


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T188284 WP_001173343.1 NZ_CP068814:c4870370-4870197 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T188284 NZ_CP090393:1866959-1867061 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT188284 WP_000398619.1 NZ_CP068814:c4870694-4870398 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT188284 NZ_CP090393:c1867068-1866923 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References