Detailed information of TA system TA188283

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4858413..4859248
• Replicon: chromosome
• Accession: NZ_CP068814
• Organism: Escherichia coli strain RIVM_C028613

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: JNN56_RS23495
• Protein ID: WP_000854821.1
• Coordinates: 4858871..4859248 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN56_RS23490
• Protein ID: WP_032216526.1
• Coordinates: 4858413..4858781 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN56_RS23450	4853536..4854420	+	885	WP_032216515.1	50S ribosome-binding GTPase	-
JNN56_RS23455	4854539..4855216	+	678	WP_032223228.1	hypothetical protein	-
JNN56_RS23460	4855222..4855455	+	234	WP_001278284.1	DUF905 family protein	-
JNN56_RS23465	4855554..4856372	+	819	WP_053902307.1	DUF945 domain-containing protein	-
JNN56_RS23470	4856464..4856949	+	486	WP_032216512.1	antirestriction protein	-
JNN56_RS23475	4856965..4857441	+	477	WP_001186724.1	RadC family protein	-
JNN56_RS23480	4857510..4857731	+	222	WP_001601167.1	DUF987 domain-containing protein	-
JNN56_RS23485	4857750..4857995	+	246	WP_201358319.1	hypothetical protein	-
JNN56_RS23490	4858413..4858781	+	369	WP_032216526.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN56_RS23495	4858871..4859248	+	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN56_RS23500	4859245..4859394	+	150	Protein_4602	hypothetical protein	-
JNN56_RS23505	4859470..4859667	+	198	WP_201358328.1	hypothetical protein	-
JNN56_RS23510	4859752..4860594	+	843	WP_032216511.1	DUF4942 domain-containing protein	-
JNN56_RS23515	4861343..4862881	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4839780..4870694	30914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T188283 WP_000854821.1 NZ_CP068814:4858871-4859248 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T188283 NZ_CP090393:833943-834353 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13810.69 Da
• Isoelectric Point: 6.2066

>AT188283 WP_032216526.1 NZ_CP068814:4858413-4858781 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLLCTVTPCFGARLVQEGNRLHYLVDRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188283 NZ_CP090393:833696-833962 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54