Detailed information of TA system TA188275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3593718..3594423
• Replicon: chromosome
• Accession: NZ_CP068814
• Organism: Escherichia coli strain RIVM_C028613

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JNN56_RS17545
• Protein ID: WP_000539521.1
• Coordinates: 3593718..3594104 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JNN56_RS17550
• Protein ID: WP_001280945.1
• Coordinates: 3594094..3594423 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN56_RS17525	3589722..3590348	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JNN56_RS17530	3590345..3591460	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
JNN56_RS17535	3591571..3591954	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JNN56_RS17540	3592167..3593492	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JNN56_RS17545	3593718..3594104	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN56_RS17550	3594094..3594423	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JNN56_RS17555	3594493..3595821	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
JNN56_RS17560	3595829..3598177	-	2349	WP_001550886.1	EAL domain-containing protein	-
JNN56_RS17565	3598355..3599266	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T188275 WP_000539521.1 NZ_CP068814:3593718-3594104 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T188275 NZ_CP090389:c4723816-4723532 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGTGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT188275 WP_001280945.1 NZ_CP068814:3594094-3594423 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT188275 NZ_CP090389:c4724048-4723806 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGTGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6