Detailed information of TA system TA188274

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3249109..3249953
• Replicon: chromosome
• Accession: NZ_CP068814
• Organism: Escherichia coli strain RIVM_C028613

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: JNN56_RS15845
• Protein ID: WP_000854686.1
• Coordinates: 3249109..3249492 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: JNN56_RS15850
• Protein ID: WP_001285602.1
• Coordinates: 3249573..3249953 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN56_RS15805	3244111..3244602	-	492	WP_032145787.1	DUF1097 domain-containing protein	-
JNN56_RS15810	3244704..3245258	-	555	WP_001001909.1	molecular chaperone YcdY	-
JNN56_RS15815	3245282..3246019	-	738	WP_000283664.1	zinc-binding phosphatase	-
JNN56_RS15820	3246074..3247012	-	939	WP_001550942.1	glyoxylate/hydroxypyruvate reductase GhrA	-
JNN56_RS15830	3247483..3248325	-	843	WP_001431817.1	DUF4942 domain-containing protein	-
JNN56_RS15835	3248410..3248607	-	198	WP_000839250.1	DUF957 domain-containing protein	-
JNN56_RS15840	3248624..3249112	-	489	WP_001054233.1	hypothetical protein	-
JNN56_RS15845	3249109..3249492	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
JNN56_RS15850	3249573..3249953	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN56_RS15855	3249964..3250647	-	684	WP_000086768.1	hypothetical protein	-
JNN56_RS15860	3250666..3250887	-	222	WP_000692298.1	DUF987 domain-containing protein	-
JNN56_RS15865	3250950..3251426	-	477	WP_001186726.1	RadC family protein	-
JNN56_RS15870	3251442..3251927	-	486	WP_000214307.1	antirestriction protein	-
JNN56_RS15875	3252020..3253591	-	1572	WP_000381395.1	IS66 family transposase	-
JNN56_RS15880	3253611..3253958	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
JNN56_RS15885	3253958..3254635	-	678	WP_032147602.1	IS66-like element accessory protein TnpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T188274 WP_000854686.1 NZ_CP068814:c3249492-3249109 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T188274 NZ_CP090389:c4637556-4637224 [Klebsiella pneumoniae]
ATGAAAACTTTACCTGCAACAACTCCGCAGACGGCGAAGCTATGTCTGACGTCCGCGGATACCTGGCAAATGTTACTGAC
ACGTCTGCTAGAACAGCATTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCAGGGATCCCTCTGGCCGATGCAGTTAACTTTCTGGTGGATAAGTACGCACTGGTTCGTATCGATCGCAGAGGATTG
AGTTGGCAGGAGCAATCATCCTATCTCCGGCTTGTCGATACTCAGCGAACCATTAAGGTTATTGAGTTGTGGCTATTTGG
TGTAATGATGTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT188274 WP_001285602.1 NZ_CP068814:c3249953-3249573 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT188274 NZ_CP090389:c4637894-4637577 [Klebsiella pneumoniae]
ATGAGCAACATCACTTGGGGCCTGCAGCGGGATATCACGCCGTGCCTGGGATCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGAGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGTTGACCACTGGTGAAATGAACCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTCCGGCTACGTATACATCGCTGTTTATCTCACCCAACGCTAA