Detailed information of TA system TA188257

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 967764..968599
• Replicon: chromosome
• Accession: NZ_CP068814
• Organism: Escherichia coli strain RIVM_C028613

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PMM7
• Locus tag: JNN56_RS04605
• Protein ID: WP_000854720.1
• Coordinates: 967764..968141 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN56_RS04610
• Protein ID: WP_126709063.1
• Coordinates: 968231..968599 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN56_RS04575	963724..964989	-	1266	WP_001218869.1	integrase arm-type DNA-binding domain-containing protein	-
JNN56_RS04580	965453..965779	-	327	WP_000779482.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JNN56_RS04585	965776..966039	-	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
JNN56_RS04590	966111..966977	-	867	WP_001290180.1	DUF4942 domain-containing protein	-
JNN56_RS04595	967070..967267	-	198	WP_000839238.1	DUF957 domain-containing protein	-
JNN56_RS04600	967279..967767	-	489	WP_021535504.1	hypothetical protein	-
JNN56_RS04605	967764..968141	-	378	WP_000854720.1	TA system toxin CbtA family protein	Toxin
JNN56_RS04610	968231..968599	-	369	WP_126709063.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN56_RS04615	968679..968900	-	222	WP_000692349.1	DUF987 domain-containing protein	-
JNN56_RS04620	968987..969463	-	477	WP_001186725.1	RadC family protein	-
JNN56_RS04625	969479..969964	-	486	WP_000849596.1	antirestriction protein	-
JNN56_RS04630	970019..970837	-	819	WP_112037995.1	DUF945 domain-containing protein	-
JNN56_RS04635	970937..971170	-	234	WP_001119719.1	DUF905 family protein	-
JNN56_RS04640	971256..971660	+	405	WP_001539664.1	IS66 family insertion sequence hypothetical protein	-
JNN56_RS04645	971657..972004	+	348	WP_000612617.1	IS66 family insertion sequence element accessory protein TnpB	-
JNN56_RS04650	972053..973591	+	1539	WP_001539662.1	IS66-like element ISEc22 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	iucC / iucB / iucA / papI	941406..972004	30598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14089.02 Da
• Isoelectric Point: 7.9087

>T188257 WP_000854720.1 NZ_CP068814:c968141-967764 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T188257 NZ_CP090386:c960544-960257 [Vibrio cholerae]
ATGACCTATAAGTTAAAGTTTCTGCCAGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGGTTAGAAAATCCTCATGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGACGAGATAGTTGTCTATGTCCTTGCTGTCGGCAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTTGGTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13574.37 Da
• Isoelectric Point: 5.5263

>AT188257 WP_126709063.1 NZ_CP068814:c968599-968231 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYPLDQAFPLLMKQLELMLTSN
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188257 NZ_CP090386:c960785-960534 [Vibrio cholerae]
ATGAGACAAGTGTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACGGCTTTGTTGAATGAAGCTGA
TGGGGCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCTGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCGGACTTATCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Structures

Toxin

• AlphaFold DB: A0A829FW05