Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4480685..4481261 Replicon chromosome
Accession NZ_CP068813
Organism Escherichia coli strain RIVM_C029033

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag JNN62_RS21605 Protein ID WP_001297643.1
Coordinates 4480974..4481261 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag JNN62_RS21600 Protein ID WP_000063160.1
Coordinates 4480685..4480987 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN62_RS21585 4477281..4479431 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
JNN62_RS21590 4479481..4479684 + 204 WP_000467859.1 YbdD/YjiX family protein -
JNN62_RS21595 4479695..4480651 + 957 WP_001297640.1 GTPase -
JNN62_RS21600 4480685..4480987 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
JNN62_RS21605 4480974..4481261 - 288 WP_001297643.1 BrnT family toxin Toxin
JNN62_RS21610 4481460..4482157 - 698 Protein_4228 IS1 family transposase -
JNN62_RS21615 4482472..4483704 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
JNN62_RS21620 4483745..4485025 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4481460..4481753 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T188249 WP_001297643.1 NZ_CP068813:c4481261-4480974 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T188249 NZ_CP090382:c2746240-2745953 [Acinetobacter towneri]
ATGACCTATAAGTTAGACTTTTTAGAAGAAGCATTAGCAGAATGGAATAAGCTTAATCCAAGTATTAAGCAGCCATTAAA
GAAGAAGTTGATCAAGGTTTTGGAGAATCCTCGCATTCCCAAAAACAACTTGTCAGGACATCCTAACCGCTACAAGATCA
AACTTCGTAGTATTGGTTATCGTTTGGTTTATGAAGTCATTGATGATGAGGTAGTTGTCATCGTGATTGCTGTAGGCAGA
CGTGAAAACAATGCCGTCTATGATGATGCCAACTCTCGTCATTCATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT188249 WP_000063160.1 NZ_CP068813:c4480987-4480685 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT188249 NZ_CP090382:c2746478-2746230 [Acinetobacter towneri]
ATGACTCAATTAATCCATGCACGAATGACTGCAAGTATTACTGAACTCAAAAAGAGTCCGATGGATACAGTGTTGGCAGG
CAAAGGTGAACCTGTTGCAATCCTCAACCGTAATACACCAGCGTTTTACTGTGTTCCGGCTGATCTGTATGAAACGATGA
TGGAACAACTAGAAGACCTGGAACTGAATAAGATTGCAGATACTCGTGCGAATCAAAAACGCATTCGAGTAAATATTGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References