Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4301208..4301826 Replicon chromosome
Accession NZ_CP068812
Organism Escherichia coli strain RIVM_C029020

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JNN61_RS20460 Protein ID WP_001291435.1
Coordinates 4301208..4301426 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JNN61_RS20465 Protein ID WP_000344800.1
Coordinates 4301452..4301826 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN61_RS20425 4296497..4297069 + 573 WP_000779831.1 YbaY family lipoprotein -
JNN61_RS20430 4297100..4297411 - 312 WP_000409911.1 MGMT family protein -
JNN61_RS20440 4297790..4298143 + 354 WP_001550741.1 DUF1428 family protein -
JNN61_RS20445 4298185..4299735 - 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JNN61_RS20450 4299899..4300369 - 471 WP_000136192.1 YlaC family protein -
JNN61_RS20455 4300485..4301036 - 552 WP_000102564.1 maltose O-acetyltransferase -
JNN61_RS20460 4301208..4301426 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JNN61_RS20465 4301452..4301826 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JNN61_RS20470 4302372..4305521 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
JNN61_RS20475 4305544..4306737 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T188222 WP_001291435.1 NZ_CP068812:c4301426-4301208 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T188222 NZ_CP090375:c970771-970583 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT188222 WP_000344800.1 NZ_CP068812:c4301826-4301452 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT188222 NZ_CP090375:c971059-970799 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References