Detailed information of TA system TA188221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4096245..4097934
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: JNN61_RS19510
• Protein ID: WP_000365809.1
• Coordinates: 4097014..4097934 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: JNN61_RS19505
• Protein ID: WP_001550661.1
• Coordinates: 4096245..4097027 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS19480	4091554..4091712	-	159	Protein_3799	integrase	-
JNN61_RS19485	4091804..4091906	-	103	Protein_3800	helix-turn-helix domain-containing protein	-
JNN61_RS19490	4091903..4092373	-	471	WP_000201185.1	hypothetical protein	-
JNN61_RS19495	4092419..4092559	-	141	WP_001308408.1	ash family protein	-
JNN61_RS19500	4092720..4095929	-	3210	WP_060624753.1	RNA-directed DNA polymerase	-
JNN61_RS19505	4096245..4097027	+	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
JNN61_RS19510	4097014..4097934	+	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JNN61_RS19515	4098368..4099252	-	885	WP_001119037.1	LysR family transcriptional regulator	-
JNN61_RS19520	4099517..4100575	+	1059	WP_122987197.1	alpha/beta hydrolase	-
JNN61_RS19525	4100717..4101844	+	1128	WP_001550663.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T188221 WP_000365809.1 NZ_CP068812:4097014-4097934 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T188221 NZ_CP090370:c1567487-1567194 [Erythrobacter sp. SDW2]
ATGCATGATGTCCTGGTGCTGCCGTCAGCACAGGCCGACCTCGATGACATCGCCATCTACACCAAGGAACAATGGGGTCG
GGACCAGGCACGACGCTACTTGGCGACGATGCGATCCGACATAACCGGCCTGGCACGCTTTCCGCGGATGCATCCAGCCT
ATGAAAGTCGCCACGGGAGTTTCCGCAAGATGTCGAGCGGAGAGCATCTTGTCTTTTTGCTCGTGAATGAGACCACGGTT
GTGGTGGTCCGGGCGCTGCACAACCGAATGGATGTCGAGGAAGCACTGGCATAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT188221 WP_001550661.1 NZ_CP068812:4096245-4097027 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT188221 NZ_CP090370:c1567731-1567480 [Erythrobacter sp. SDW2]
ATGACAGCCAAGAATACCTCTATCGCCCTCGGCAAACCCTATACCGATTTTGCCCGCCGCAAGGTCGAAAGCGGCGAATT
CGCCACCACCAGCGAAGTCGTGCGCGAAGCGATGCGGCAGTACATCGCGCAGGACGCGAAGCGCGAGGCCTTGGACCGGG
CACTGAAGGAAGGGCTCGACAGCGGCCCGGCGCGTCCGTTCGACTGGGATGCCTTCATGGGCCGGCATTTCGGACCCGGC
GAAGATGCATGA

Structures

Toxin

• AlphaFold DB: V0Z9M0